人骨形成蛋白-7基因转染犬骨髓基质细胞促进牙周组织再生的实验研究

Effect of hBMP-7 gene modified bone marrow stromal cells on periodontal tissue regeneration

目的 探讨人骨形成蛋白-7（Hbmp-7）基因修饰的骨髓基质细胞（BMSC）在牙周组织再生中的作用,为基因治疗与组织工程联合应用于牙周组织缺损再生治疗奠定基础.方法 人工构建5只Beagle犬慢性Ⅱ度根分叉病变模型,将转染Hbmp-7基因的BMSCs与未转染BMSCs分别以1×107个/ml接种于胶原膜BME-10X,植入牙周缺损内,对照组仅植入胶原膜,12周后HE染色观察牙周组织再生情况.结果 各实验组和对照组均可见不同程度的牙周组织再生,实验组新生牙槽骨面积百分比和新生牙骨质长度百分比与对照相比有明显增加（P〈0.05）;转染细胞复合胶原膜组新生牙槽骨面积百分比（81.8%±7.8%）与未转染细胞复合胶原膜组（67.3%±10.3%）相互比较差异有统计学意义（P〈0.05）;而其新生牙骨质长度百分比（79.1%±8.6%与75.6%±7.3%）比较差异无统计学意义（P〉0.05）.结论 Hbmp-7基因强化的组织工程技术是修复牙周组织缺损的一种较好的方法,有很好的临床应用前景.

Objective To estimate the potential effects of hBMP-7 gene transfected bone manow stromal cells （BMSC） and to provide a new way for repairing the periodontal defects. Methods A total of 30 experimental class Ⅱ furcation defects in five Beagle dogs were created surgically. The transfected and non-transfected BMSC were seeded in collagen membrane at an initial concentration of 1×107/ml and transplanted into experimental class Ⅱ furcation defects. The animals were sacrificed at 12 weeks post-operation and periodontal regeneration was evaluated by the histological and morphometric analysis. Results In both experimental and control groups, there were different extents of regeneration. The percentage of new alveolar area in transfected and non-transfected BMSC were significantly higher than the control（P〈0. 05）. And there was also significant difference between two experimental groups （P〈0. 05）. The percentage of new cementum length in two experimental groups were significantly higher than the control（P〈0. 05）. But there was no significant difference between two BMSCs groups （P〉0. 05） . Conclusion hBMP-7 gene enhanced tissue enginccring could be a betler way to repair the perlodntal defeits.