胃癌组织中基质金属蛋白酶-2和胰岛素样生长因子-1蛋白表达及其意义

Expression of matrix metalloproteinase-2 and insulin-like growth factor-1 in gastric carcinoma and their clinicopathological significance

目的 探讨基质金属蛋白酶-2（MMP-2）和胰岛素样生长因子-1（IGF-1）两种蛋白在胃癌组织中的表达及其临床病理意义.方法 应用免疫组化方法检测436例胃癌组织和92例非肿瘤胃黏膜组织中MMP-2和IGF-1蛋白的表达情况,分析MMP-2和IGF-1与胃癌浸润、转移和预后之间的关系.结果 胃癌组织中MMP-2和IGF-1的高表达率分别为70.4%（307/436）和49.5%（216/436）,明显高于非肿瘤胃黏膜组织中的3.3%（3/92,P〈0.05）和5.4%（5/92,P〈0.05）,两种蛋白在胃癌组织中的表达水平均与肿瘤患者的年龄、肿瘤大小、肿瘤部位、Lauren分型、TNM分期、浸润深度、脉管侵犯、远处转移和淋巴结转移存在相关性（P值均〈0.05）;MMP-2和IGF-1两种蛋白的表达呈正相关（P〈0.05）;按TNM分期的单因素生存分析表明,MMP-2高表达与Ⅰ～Ⅱ期胃癌患者的预后不良相关（P值均〈0.05）,IGF-1高表达与Ⅰ～Ⅲ期胃癌患者的预后不良有关（P值均〈0.05）;MMP-2和IGF-1的高表达均为胃癌的独立预后因素.结论 MMP-2和IGF-1参与了胃癌的侵袭、转移过程,同时检测MMP-2和IGF-1两种蛋白的表达有助于判断胃癌患者预后.

Objective To investigate the expression of matrix metalloproteinase-2 （MMP-2） and insulin-like growth factor-1 （IGF-1） in gastric carcinoma and their clinicopathological significance. Methods Expressions of MMP-2 and IGF-1 were examined by using immunohistochemical SP staining and cross- compared with clinicopathological features of gastric carcinoma. Results High expression of MMP-2 and IGF-1 were observed in 70.4% （307/436） and 49. 5% （216/436） of gastric carcinoma tissues respectively, significantly higher than those in non-tumor gastric mocosa（3. 3% and 5.4% , respectively; all P 〈0.05）. The high expression rate of MMP-2 and IGF-1 were significantly associated with the patient age, tumor size, tumor location, Lauren classification, TNM staging, depth of tumor infiltration, presence of vessel invasion, lymph node and distant metastasis （ all P 〈 0. 05 ）. In addition, the expression of MMP-2 was positively linked with the expression level of IGF-1 （P〈0.05）. Univariate analysis showed that high expression of MMP-2, was significantly associated with poor prognosis of tumor of TNM stage I and II （ all P 〈 0. 05 ） , high expression of IGF-1 was significantly correlated with poor prognosis of patients with TNM stage Ⅰ , Ⅱ and Ⅲtumor （all P 〈 0. 05）. Cox multivariate analysis indicated that the high expressions of MMP-2 and IGF-1 could be independent prognostic indices for gastric carcinoma. Conclusions High expression of MMP-2 and IGF-1 proteins are significantly correlated with the invasion and metastasis of gastric carcinoma,it is helpful to simultaneously detect the expressions of MMP-2 and IGF-1 proteins in predicting prognosis of patients with gastric carcinoma.