养阴抗毒胶囊对放射损伤大鼠肾组织热休克蛋白70与糖皮质激素受体表达的影响

The Effect of Yin Nourishing and Antitoxic Capsule on the expression of Heat shock protein 70 and GR in kidney tissues in rats with radiation injury by X-ray

目的:研究养阴抗毒胶囊对放射性损伤大鼠肾热休克蛋白70(HSP70)与糖皮质激素受体的表达的影响及作用机制。方法:40只雄性SD大鼠随机分成4组,即正常对照组(A)、放射对照组(B)、放射+养阴抗毒组(C)和放射+桂附地黄组(D),以6GyX射线照射大鼠造成重度造血型放射损伤模型,用蛋白印迹法(Western Blot)检测肾组织热休克蛋白70与糖皮质激素受体(GR)的表达情况。结果:HSP70在放射对照组(B)、放射+养阴抗毒组(C)和放射+桂附地黄组(D)均有高表达,以B组最为明显,D组其次,C组最弱。而GR在C组表达最明显,其次在D组。结论:养阴抗毒胶囊能显著降低放射损伤大鼠肾组织中HSP70的表达,而桂附地黄丸的此作用相对较弱,养阴抗毒胶囊能明显提高糖皮质激素受体的表达。

Objective：To explore the protection mechanism of Yin Nourishing and Antitoxic Capsule＇s to radiation injury in rats, and the effect of Yin Nourishing and Antitoxic Capsule on expression of HSP70 and GR in kidney tissues in rats with radiation injury. Methods：Forty adult male SD rats were randomly divided into four groups with ten rats in each group： normal group（ A）, radiation group（ B）, radiation and Nourishing Yin and Antitoxic group （C）, radiation and Gui Fu Di Huang group（D） . The rats in group B group C and group D were exposed to whole - body by X - ray radiation at a dose of 6Gy. Then HSP70 and GR expression in kidneys of all groups was tested with Western blot method. Results： HSP 70 had apparent expression in group B, group C and group D, with highest expression in group B ,followed by group D, and lowest in group C. While GR had obviously higher expression in group C, and group D ranked second. Conclusion ： Yin Nourishing and Antitoxic Capsule can significantly reduce the HSP 70 expression in kidneys of radiation injury by X - ray in rats, but Gui Fu Di Huang pills have less function. Yin Nourishing and Antitoxie Capsule can improve GR expression of radiation injury by X - ray in rats.