微小RNA对人舌鳞状细胞癌Tca8113细胞增殖的影响

Effect of microRNA on proliferation of human tongue carcinoma Tca8113 cells

目的 探讨微小RNA（microRNA,miRNA）对体外培养的人舌鳞状细胞癌TeaS113细胞增殖的影响.方法 用脂质体把miRNA-31和miRNA-139的类似物或抑制物分别导人人舌鳞状细胞癌Tca8113细胞,分别为实验组,脂质体试剂为对照组,然后用甲基噻唑基四唑（MTT）法检测细胞增殖情况.结果 对照组吸光度值（A值）在24、48和72 h时分别为（0.125±0.002）、（0.169±0.002）和（0.216 4±0.004）;miRNA-31类似物可促进Tca8113细胞增殖,使其A值分别增至（0.136±0.001）（P〈0.001）、（0.186±0.004）（P〈0.001）和（0.249±0.012）（P〈0.01）;miRNA-139抑制物也使A值分别增加到（0.148±0.002）（P〈0.001）、（0.214±0.002）（P〈0.001）和（0.250±0.009）（P〈0.01）.与此相反,miRNA-31抑制物在48、72 h时抑制Tea8113细胞增殖,其A值分别降至（0.145±0.001）和（0.155±0.011）（P〈0.001）;mjRNA-139类似物在24、48 h也分别使A值降至（0.135±0.001）和（0.170±0.009）（P〈0.001）.结论 miRNA-31和miRNA-139在人舌鳞状细胞癌发生过程中起着重要作用,调整其活性有可能成为一种有效治疗口腔癌的新方法.

Objective To investigate the effects of microRNA（miRNA）on proliferation of cultured human squamous cell carcinoma of tongue Tca8113 cells.Methods The mimics or inhibitors of miRNA-31 or miRNA-139 were transfected into Tca8113 cells using liposome.Tca8113 cell proliferation was detected by 3-（4,5-dimethyhhiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay.Results The absorbance （A） values of control group at 24 h,48 h and 72 h were 0.125±0.002,0.169±0.002 and 0.216±0.004.respectively.The mimics of miRNA-31 increased Tca8113 cell proliferation.with A values inereasing to 0.136±0.001（P〈0.001）.0.186±0.004（P〈0.001）and 0.249±0.012（P〈0.01）,respectively.The inhibitors of miRNA-139 also inereased A values to 0.148±0.002（P〈0.001）.0.214±0.002（P〈0.001）and 0.250±0.009（P〈0.01）,respectively.Contrast with these results,the inhibitors of miRNA-31 decreased Tca8113 cell proliferation,with A values decreasing to 0.145 4-0.001 and 0.155±0.011 （both of P〈0.001） at 48 h and 72 h,respectively.The mimics of miRNA-139 also decreased A to 0.135±0.001 and 0.170±0.009 （both of P〈0.001）.Conclusions miRNA-31 and miRNA-139 play an important role in the carcinogenesis of human tongue carcinomas.It may become a new method for the treatment of tongue carcinomas by adjustment the activities of miRNA.