肽段-量子点对鳞状细胞癌细胞成瘤及淋巴转移能力影响的动物实验

Effect of peptide-conjugated quantum dots on the tumorigenicity and lymph node metastasis of human tongue squamous cell carcinoma cell line Tca8113 and mouse uterine cervix carcinoma U14 in vivo

目的观察肽段连接的最大发射波长为655nm的荧光量子点（quantumdots，QD655）对人舌鳞状细胞癌（Tea8113）和昆明小鼠淋巴高转移鳞状细胞癌（U14）的体内生长、增殖、凋亡和淋巴转移能力的影响。方法①用QD655分别标记Tea8113细胞（Tea8113-QD655）和U14细胞（U14-QD655），将Tea8113-QD655和U14-QD655分别接种于裸鼠和昆明小鼠背部皮下，比较Tea8113-QD655和Tea8113、U14-QO655和U14的体内成瘤情况，并用流式细胞仪检测比较体内成瘤的Tea8113-QD655和Tea8113细胞、U14-QD655和U14细胞的增殖和凋亡情况；②将U14-QD655和U14分别接种于昆明小鼠颊黏膜下，建立颊癌颈淋巴转移模型，比较U14-QD655和U14颈淋巴转移能力的变化。结果Tea8113-QD655组和Tea8113组肿瘤的平均重量分别为（1．25±0．14）、（1．30±0．16）g（P〉0．05），肿瘤的平均体积分别为（2．81±0．68）、（2．69±0．63）cm。（P〉0．05）；U14-QD655组和U14组肿瘤的平均重量分别为（1．17±0．08）、（1．22±0．10）g（P〉0．05），肿瘤的平均体积分别为（2．27±0．56）、（2．38±0．61）cm^3（P〉0．05）。Tea8113-QD655和Tca8113体内成瘤细胞的平均增殖指数分别为（47．42±1．71）％、（48．33±1．52）％（P〉0．05），平均凋亡指数分别为（12．38±0．75）％、（11．79±0．64）％（P〉0．05）；U14-QD655和U14体内成瘤细胞的平均增殖指数分别为（61．78±2．41）％、（60．94±2．26）％（P〉0．05），平均凋亡指数分别为（13．65±0．91）％、（12．78±0．83）％（P〉0．05）。U14-QD655颊癌和U14颊癌的颈淋巴结转移率分别为41％（11／27）、43（13／30）（P〉0．05）。结论用量子点标记Tca8113和U14后不影响体内肿瘤生长和淋巴转移能力。

Objective To observe the effect of peptide-conjugated quantum dots with a maximal emission of 655 nm（ QD655 ） on growth, proliferation, apoptosis and lymphatic metastasis of human tongue squamous cell carcinoma cell line Tea8113 and mouse uterine cervix carcinoma U14 in vivo. Methods Tca8113 and U14 cells were labeled by QD65s （TcaSl13-QD655, U14-QD655 ）. TcaSl13-QD65s and U14-QD655 were inoculated subcutaneously into nude mice and Kunming mice. The tumor formation of TcaS113-QD6ss and TcaS113,U14-QD6ss and U14 was observed and compared in vivo. The proliferation and apoptosis of TcaS113-QD655 and Tca8113, U14-QD655 and UI4 cells from tumors formed in vivo were analyzed by flow cytometry. U14-QD655 and U14 were inoculated into the buccal mucosa of Kunming mice to establish the cervical lymph node metastasis model of buccal cancer. The cervical lymph node metastatic ability of U14-QD655 and U14 was compared. Results The tumor weight and volume of Tca8113-QD655 and Tca8113, U14-QD655 and UI4 in vivo were not significantly different （ P 〉 0. 05 ） , the cell proliferation index and apoptosis index of Tca8113-QD655 and Tca8113, U14-QD655 and U14 in vivo were not significantly different （P 〉0. 05）. The cervical lymph node metastasis rate of U14-QD655 and U14 buccal cancer were not significantly different（P 〉 0. 05 ）. Conclusions QD showed no effects on tumorigenicity and lymph node metastasis of Tea8113 and U14 cells. These results provide the scientific basis for noninvasive imaging and long-term tracing study.