重组人血管内皮抑素联合顺铂对S180肉瘤VEGF和MMP-9表达的影响

The effect of recombinant human endostatin combined with cisplatin on the expression of VEGF and MMP-9 of C57BL/6 mouse with sarcoma

目的观察重组人血管内皮抑素(恩度)联合顺铂对S180肉瘤C57BL/6鼠模型以及小鼠肿瘤组织中血管内皮生长因子(VEGF)和基质金属蛋白酶-9(MMP-9)表达的影响。方法建立S180肉瘤C57BL/6鼠模型,成瘤后随机分为对照组、恩度组、顺铂组、顺铂+恩度联合组,检测肿瘤组织中VEGF及MMP-9的表达。结果成瘤后对照组、恩度组、顺铂组、顺铂+恩度联合组中的VEGF及MPP-9表达H评分分别为51.4±2.5、48.4±5.3、41.4±5.2、32.3±3.9和47.2±3.6、46.1±6.1、36.5±6.4、31.3±3.2;与对照组比较,顺铂组和联合组均有显著差异(P<0.05);顺铂组与联合组之间亦有显著差异(P<0.05)。结论恩度联合顺铂能够显著抑制VEGF及MMP-9的表达,减少肿瘤血管生成,从而减少肉瘤浸润及转移。

Objective To evaluate the antiangiogenic effect of recombinant human endostatin（ED）combined with cisplatin（DDP） on model of C57BL/6 rats.Methods S180 sarcoma cells were inoculated in C57BL/6 mouse.Then the mouse with sarcoma were randomized into control group,ED group,DDP group and ED/DDP group,they were treated according to the plan.And the expression of VEGF and MMP-9 were evaluated.Results After the adminstration of control group,ED group,DDP group,ED/DDP group,the H-score of VEGF and MMP-9 were 51.4±2.5,48.4±5.3,41.4±5.2,32.3±3.9;47.2±3.6,46.1±6.1,36.5±6.4,31.3±3.2,respectively.DDP group,ED/DDP group were obviously lower than control group（P0.05）.Conclusion Recombinant human endostatin combined with cisplatin can greatly inhibit the expression of VEGF and MMP-9,and reduce the recurrence,metastasis of sarcoma by antiangiogenisis.