脑蛋白水解物注射液对NG2蛋白聚糖阳性神经祖细胞分化的影响

Effects of Cerebrolysin on the differentiation of the NG2 proteoglycan-positive neural progenitor cells

目的探讨脑蛋白水解物注射液(Cerebrolysin,CL)对NG2蛋白聚糖阳性神经祖细胞(NG2细胞)分化的作用。方法根据文献从成年大鼠海马原代培养NG2细胞,7天后添加10μl/ml(0.4mg/ml)CL继续培养7天,以免疫荧光双重染色法鉴定细胞性质。结果10μl/mlCL处置明显增加NG2细胞中Map2a/b、SynapsinI、VGAD和GABA表达水平。结论CL促进NG2细胞向神经元(特别是GABA能抑制性中间神经元)分化。

Objective To investigate the effects of Cerebrolysin（CL） on the differentiation of the NG2 proteoglycan-positive neural progenitor cells （NG2 cells）. Methods NG2 cell primary culture was done from adult rat hippocampus according to the reference. On the 7d, 10μl/ml CL was added to the medium and cultured for another 7 days. The characteristics of the cells were identified by double immunofluorescence stain. Results 10μl/ml CL increased the expression level of Map2a/b, Synapsin I, VGAD and GABA in NG2 cells significantly. Conclusion CL promotes the differentiation of NG2 cells to neurons （particular GABAergic inhibitory interneurons）.