戊四氮点燃癫癎大鼠空间学习记忆改变及海马NR2B表达

NR2B expression in hippocampus with spatial learning and memory impairment after pentylenetetrazol-kindled rats

目的观察戊四氮点燃癫癎大鼠空间学习记忆功能变化及海马NMDA2型受体(NR2)B亚单位(NR2B)表达,探讨二者的关系及PTZ致癎大鼠认知障碍发生的分子机制。方法采用戊四氮(PTZ)慢性癫癎(CE)模型,Y-迷宫对两组大鼠进行行为学检测,免疫组织化学方法观察两组大鼠海马CA3区NR2B表达的变化,反转录多聚酶链反应(RT-PCR)方法检测大鼠海马NR2B mRNA的表达。结果癫癎组大鼠空间学习记忆能力受损;其海马CA3区NR2B阳性细胞较对照组明显减少(P<0.01),同时伴有海马NR2B mRNA表达下降(P<0.01)。结论戊四氮点燃癫癎大鼠空间学习记忆受损可能与海马神经元NR2B的表达减少有关。

Objective To observe the change of pentylenetetrazol ignited epileptic rats spatial learning and memory function and the expression of NMDA 2 receptor （NR2） B subunit （NMDA receptor2B, NR2B）in the hippocampus , explore the relationship between the two and the molecular mechanism for the PTZ ignited epileptic rats cognitive impairment. Methods Using model of chronic epilepsy induced by pentylenetetrazol （PTZ）, the behavior was tested by Y-maze,the expression of NR2B in hippocampus was determined by immunohistochemical and RT-PCR methods respectively . Results The learning and memory ability of the epilepsy rats was impaired, the NR2B positive cells were decreased （P0.01） and the expression level of NR2B mRNA in hippocampus was also decreased（P0.01）. Conclusion The impairment of learning and memory ability of the epilepsy rats might be related with the change of NR2B in hippocampal neurons.