甲基强的松龙对多发性硬化患者外周血IL-12和CXCL13的影响

Methylprednisolone regulate the secretion of IL-12 and CXCL13 in patients with multiple sclerosis

目的通过检测多发性硬化(MS)患者用甲基强的松龙(MP)治疗前后外周血中的白介素-12(IL-12)和趋化因子CXC配体13(CXCL13)水平的变化情况,探讨活化的T细胞和活化的B细胞分泌的细胞因子在发病机制中的作用。方法以酶联免疫吸附(ELISA)方法检测对照组及MP治疗组在治疗前后的IL-12和CXCL13水平。结果MP治疗前,MS患者的IL-12和CXCL13水平显著高于对照组(IL-12p<0.05;CXCL13p<0.01),MP治疗后与治疗前相比,MS患者的IL-12水平显著下降(p<0.05),而CXCL13水平显著升高(p<0.05)。结论MP治疗使IL-12水平降低;使CXCL13水平升高。

Objective To determine the effect of activated T cells and B cells typed cytokine in the patients with multiple sclerosis（MS）,the secretion of IL-12 and CXCL13 before and after the treatment of methylprednisolone（MP）was measured.Methods The levels of IL-12 and CXCL13 before and after the treatment of methylprednisolone were detected by ELISA in MS patients and normal controls（NC）.Results The levels of IL-12 and CXCL13 in MS group were significantly higher than those in NC group（IL-12 P0.05,CXCL13 P0.01）before the treatment of MP;the levels of IL-12 were significantly decreased but that of CXCL13 were significantly increased after the treatment of MP（P0.05）.Conclusion The MP therapy causes the increasing of IL-12 and the decreasing of CXCL13.