液晶/聚氯乙烯复合膜的表面拓扑结构调控及蛋白吸附

Surface topological structure control and protein adsorption of liquid crystal/polyvinyl chloride composite membranes

背景:蛋白质在材料表面的吸附行为对于提高材料的生物相容性起着至关重要的作用。研究证明材料表面液晶态结构的引入改善了纯基材聚合物的生物相容性。目的:利用仿生学原理,模仿生物膜表面的液晶结构形态,制备液晶/聚氯乙烯复合膜。通过不同温度的热处理诱导液晶畴的尺寸及排列,以调控液晶/聚氯乙烯复合膜表面拓扑结构。方法:称取一定量的聚氯乙烯及液晶化合物胆甾醇三缩四乙二醇碳酸酯,分别溶于四氢呋喃中配制成溶液,再按体积比混合制备不同液晶含量的液晶/聚合物复合膜,置于恒温恒湿反应箱中4h,按同样方法制备纯聚氯乙烯膜作对照。通过偏光显微镜、扫描电子显微镜观察不同液晶含量复合膜经不同温度热处理后的表面拓扑形貌,并对复合膜进行了蛋白质吸附研究。结果与结论:液晶/聚氯乙烯复合膜表面呈现出的液晶微区的形态特征受到液晶含量、热诱导作用等因素的影响。液晶含量越高,表面越光滑;随着温度增加,表观粗糙度增大。结果提示,通过适当调节温度和液晶含量可获得具有一定拓扑结构的复合膜,从而影响复合膜表面蛋白吸附。

BACKGROUND：Protein adsorption on material surface plays an important role in improving biocompatibility of materials.In addition,introducing of liquid crystal（LC） structure can amend the biocompatibility of polymer.OBJECTIVE：To prepare LC /polyvinyl chloride（PVC） composite membranes via imitating the LC structure of the surface of biological membranes based on the idea of ＂bionic design＂.METHODS：A certain amount of PVC and LC compounds were dissolved in tetrahydrofuran to prepare solutions,and to prepare LC /PVC composite membranes according to different mixture ratios.The composite membranes were placed in reaction box for 4 hours,and drying in vacuum.The pure PVC membranes were prepared as the controls.The surficial topological features of the membranes were observed under polarizing microscope and scanning electron microscope.The protein adsorption on the surfaces was studied.RESULTS AND CONCLUSION：The surficial topological structure and performance of the membranes were able to be controlled through adjustment of the size and arrangement of LC domains by thermal treatment in different temperature and change of LC contents.That is,the surficial smooth was increased with high LC contents and decreased temperature.The expected topological structure could be obtained by adjusting LC contents and temperature of thermal treatment,which affected the protein adsorption on the membrane surfaces.