壳聚糖和葡甘聚糖生物膜制备及体外性能

Preparation of chitosan/konjac glucomannan composite membranes and the in vitro performances

背景:近年来,不少学者发现将壳聚糖和葡甘聚糖共混,可改善二者单独使用某些性能的不足。目的:制备壳聚糖和葡甘聚糖复合生物膜,并评价其对人牙周膜细胞增殖的影响。方法:将壳聚糖与葡甘聚糖以不同质量比混合,采用流延法制备壳聚糖与葡甘聚糖混合膜,与第6代人脐带间充质干细胞培养评价细胞相容性和细胞毒性,测定其体内外降解性能。并观察混合膜对人牙周膜细胞的作用。结果与结论:获得壳聚糖/葡甘聚糖含量分别为3:0,2:1,1:1,1:2的8种良好组织相容性膜。体外降解实验显示,随着葡甘聚糖含量增加,壳聚糖/葡甘聚糖膜的降解率提高。在体外有溶菌酶存在时,混合膜70d降解率分别为40.75%、65.70%,74.20%和84.60%;体内降解实验显示,壳聚糖/葡甘聚糖含量比为3:0,2:1,1:1时在体内可存留12周以上。当葡甘聚糖含量占主要成分如壳聚糖/葡甘聚糖比为1:2时,可在体内存留8周。随着葡甘聚糖含量的增加,壳聚糖/葡甘聚糖混合膜对人牙周膜细胞增殖作用逐渐减弱,加载碱性成纤维细胞生长因子后,壳聚糖/葡甘聚糖含量比为3:0,2:1的混合膜可提高细胞碱性磷酸酶活性,但以2:1的混合膜最明显。结果说明所获得的混合膜初步具备了诱导组织再生膜和组织工程支架的基本性能。

BACKGROUND：Recent studies indicated that the composition of chitosan（CS） and konjac glucomannan（KGM） can improve their own biological features.OBJECTIVE：To prepare CS /KGM composite membranes,and to evaluate its effect on the proliferation of periodontal ligament fibroblast cells（PDLCs）.METHODS：Blend membranes containing CS and KGM solutions with different weight proportions were prepared by solvent-casting method.The 6th passage of human umbilical cord mesenchymal stem cells were used to observe the cell compatibility and cytotoxicity and to determine the degradation features.The effects of those cells on PDLCs were assessed.RESULTS AND CONCLUSION：Eight kinds of composite membranes containing CS and KGM（3：0,2：1,1：1,1：2） were obtained.In vitro degradation test demonstrated that the degradation rates of CS /KGM composite membranes were increased with the adding of KGM,which were 40.75%,65.70%,74.20% and 84.60% respectively at 70 days.In vivo test showed that the CS /KGM composite membranes could maintain more than 12 weeks when at the CS /KGM ratios of 3：0,2：1 and 1：1.If the CS /KGM ratio was 1：2,the CS /KGM composite membranes could maintain 8 weeks in vivo.The effects of the composite membranes on human PDLCs were decreased with increasing of KGM contents.After adding basic fibroblast growth factor,the CS /KGM composite membranes with ratios of 3：2 and 2：1 could increase the alkaline phosphatase activities,especially with ratio of 2：1.Results from the study suggested that CS /KGM membrane is a potential periodontal tissue engineering scaffold.