逆转录病毒介导myostatin在鸡胚胎中的过表达与检测

Over-expression and Detection Analysis of Myostatin in Chicken Embryos Mediated by Retrovirus

鸡的胚胎已经成为发育生物学、免疫学和癌症等学科的重要实验模型之一。以鸡胚胎为实验模型,以鸡myostatin为靶基因,利用逆转录病毒介导和原位杂交技术相结合的方法来研究目的基因的鸡胚胎转导和检测分析。实验结果表明:逆转录病毒介导的目的基因myostatin能够有效整合到胚胎基因组中,且具有转录活性;胚胎原位杂交检测能够准确直观的反映病毒的整合部位和转入基因的表达水平。不但为进一步研究鸡myostatin基因在胚胎发育中的功能奠定基础,同时在方法上也进行了简单的优化尝试。

Chick embryos have become a great experimental model system for developmental biology,immunology and cancer,etc.The chicken embryo and myostatin are used as model system and target gene respectively.The process of transferring gene into chicken embryo and detection are researched using retrovirus-mediated gene transfer and in situ hybridization.The experimental results show that target gene myostatin can be integrated into the embryonic genome,and has transcriptional activity;Whole-mount in situ hybridization can accurately reflect the virus integration sites and target gene expression levels.The study not only provides a base for further study myostatin functions in embryonic development,but also is a methodology attempt.