IGF-1调控乳腺癌VEGF-C表达的实验研究

The study of IGF-1 in stimulating expression of vascular endothelial growth factor-C in the breast cancer cell line MDA-MB-231

目的:探讨胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)对乳腺癌MDA-MB-231细胞株的血管内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)表达的调控及其相关信号传导通路。方法:应用实时定量PCR和Western印迹技术检测IGF-1刺激前后目的基因VEGF-C mRNA及其蛋白在乳腺癌细胞中表达的变化,检测细胞内相关信号传导分子Akt、ERK1/2蛋白及其磷酸化表达改变。结果:IGF-1显著促进乳腺癌细胞株的VEGF-C表达(P<0.05);VEGF-C表达与IGF-1间呈浓度依赖关系;在IGF-1作用下,p-Akt及p-ERK1/2表达显著增加(P<0.05)。结论:IGF-1能显著促进乳腺癌MDA-MB-231细胞株的VEGF-C表达,信号传导分子p-Akt与p-ERK1/2可能在其中起着重要的介导作用。

Objective To study the pathway between IGF-1 and VEGF-C in the breast cancer cell line MDA-MB-231. Methods Recombinant Human IGF-1 was used to stimulate VEGF-C in the breast cancer cell line MDA-MB-231. VEGF-C mRNA was detected by Real-Time Quantitative PCR; VEGF-C protein, ERK protein and AKT protein were detected by Western blotting. Results the expression of VEGF-C mRNA and VEGF-C protein in the breast cancer cell line was significantly increased;The expression of p-AKT and p-ERK1/2 were also increased after stimulating the cell line using the cytokine of IGF-1. Conclusions IGF-1 may increase the expression of VEGF-C in the breast cancer cell line of MDA-MB-231. ERK and AKT may play important roles in this process.