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妊娠早期CD56^(bright)CD16^- NK细胞CD49d和CD11b的表达分析 被引量：2

Expressions of CD49d and CD11b on the CD56^(bright)CD16^- NK cell of early pregnancy
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摘要 目的探讨早期妊娠和稽留流产患者外周血CD56brightCD16- NK细胞及蜕膜组织子宫自然杀伤(uNK)细胞中整合素α4(CD49d)和αm(CD11b)的表达。方法采集18例早期妊娠妇女外周血(其中14例另取蜕膜组织)和9例稽留流产妇女外周血,Ficoll密度梯度离心法分离单个核细胞悬液,流式细胞术检测样本CD56brightCD16- NK细胞中CD49d和CD11b表达。结果 CD49d和CD11b在早期妊娠蜕膜CD56brightCD16- NK细胞表达显著低于早期妊娠妇女外周血CD56brightCD16-NK细胞(P<0.01);稽留流产患者外周血CD56brightCD16- NK细胞的表达较早期妊娠妇女显著降低(P<0.05)。结论 CD49d和CD11b可能在妊娠早期uNK细胞的募集中起重要作用,同时也可能参与了正常妊娠的维持。 Objective To investigate expressions of integrinα4（CD49d）and integrinαm（CD11b）on the CD56brightCD16- NK cell of the peripheral blood and decidua in early pregnant women and the peripheral blood in missed abortion women.Methods Peripheral blood samples were collected from early pregnant and missed abortion females,and uNK cells were isolated from the fresh decidua of early pregnant females by mechanical trituration and Ficoll gradient centrifugation.The fluorescent intensity of the integrines was detected by FCM.Results Expressions of CD49d and CD11b on the CD56brightCD16- NK cell of peripheral blood in early pregnant women were higher in the first trimester decidua（P0.01）,and also higher than those in missed abortion women（P0.05）.Conclusion CD49d and CD11b may play an important role in the recruitment of uNK cells and participates in the maintenance of normal pregnancy.
作者 黄先亮 董白桦 胡微煦 王庆杰 宋丙凤 王春娥 刘佳 孔北华 曲迅
机构地区 山东大学齐鲁医院妇产科 山东大学齐鲁医院临床基础研究所
出处 《山东大学学报（医学版）》 CAS 北大核心 2010年第5期97-100,共4页 Journal of Shandong University:Health Sciences
基金 国家自然科学基金资助课题(3087232130671902) 山东省自然科学基金资助课题(Y2008C02)
关键词 早期妊娠 稽留流产 UNK细胞 整合素 Early pregnancy Missed abortion uNK cell Integrin
分类号 R714.14 [医药卫生—妇产科学]
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参考文献8

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同被引文献14

  • 1Santoni A, Zingoni A, Cerboni C, et al. Natural killer (NK) cells from killers to regulators: distinct features be- tween peripheral blood and decidual NK cells [ J ]. Am J Reprod Immunol, 2007, 58(3 ) :280.
  • 2Hanna J, Goldman-Wohl D, Hamani Y, et al. Decidual NK cells regulate key developmental processes at the hu- man fetal-maternal interface [ J]. Nat Med, 2008, 14 (11) :1184-1185.
  • 3Kairbaan, Hodivala-Dilke. αvβ3 integrin and angiogene- sis: a moody integrin in a changing environment[ J]. Curr Opin Cell Biol, 2008, 20(5) :514-519.
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  • 5Burrows T D, King A, Loke Y W. The role of integrins in adhesion of decidual NK cells to extracellular matrix and decidual stromal cells [ J]. Cell Immunol, 1995, 166(1) :53-61.
  • 6Mangale S S, Modi D N, Reddy K V R. Identification of genes regulated by an interaction between αvβ3 inte- grin and vitronectin in murine decidua[ J ]. Reprod Fertil Dev, 2008, 209(2) :311-319.
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  • 9Casals G, Ordi J, Creus M, et al. Osteopontin and αvβ3 integrin expression in the endometfium of infertile and fer- tile women[J]. Reprod Biomed Online, 2008, 16(6) : 808-816.
  • 10Hu Y, Eastabrook, Tan R, et al. Decidual NK cell-de- rived conditioned medium enhances capillary tube and net- work organization in an extraviUous cytotrophoblast cell line[J]. Placenta, 2010, 31(3):213-221.

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山东大学学报（医学版）
2010年 第5期

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