一氧化氮和氧自由基对培养大鼠皮层神经细胞的损伤

Damage to cultured rat cortical cells due to nitric oxide and superoxide

目的：探讨一氧化氮（ＮＯ）和氧自由基（Ｏ－２）对培养大鼠皮层神经细胞的损伤作用及两种损伤因素间的关系。方法：应用原代大鼠皮层神经细胞培养方法，研究外源性损伤因素的作用。结果：①以硝普钠（ＳＮＰ）为ＮＯ供体的损伤组较对照组的乳酸脱氢酶（ＬＤＨ）释放量显著增高，而细胞存活率显著降低，加入ＳＯＤ后ＳＮＰ的损伤作用减轻。②Ｏ－２损伤组的结果与ＮＯ损伤组结果相似，同时应用Ｏ－２和ＮＯ组对神经细胞的损伤作用显著高于单独应用Ｏ－２组或ＮＯ组。结论：ＮＯ和Ｏ－２都能造成培养的大鼠皮层神经细胞损伤。

Objective:To study the damage to cultured rat cortical cells due to nitric oxide (NO) and superoxide (O - 2),and to explore the relationship between NO and O - 2.Methods:Using primary cortical cell cultures to study damaging factors.Results:①Compared with control group,the Lactate Dehydrogenase (LDH) release caused by SNP (the provider of NO) increased significantly ( P <0 01),and the survival rate decreased significantly ( P <0 01).②The results of O - 2 damage to neuron were similar to those of SNP.More serious damage was caused by using NO and O - 2 together than by using NO or O - 2 separately ( P <0 01).Conclusion:Both NO and O - 2 can cause damage to cultured rat cortrical cells,and there is cooperation between them.