β_1整合素及局部粘附激酶P^(125FAK)在人肺癌细胞中的表达及其意义

The expression of β1 integrin and focal adhesion kinase P 125FAK on human lung cancer cell lines

目的：探讨β１整合素和局部粘附激酶Ｐ１２５ＦＡＫ在人肺癌细胞粘附和转移过程中的作用。方法：用细胞粘附实验检测β１整合素在１０种人肺癌细胞粘附中的作用；用Ｗｅｓｔｅｒｎ印迹检测β１整合素和局部粘附激酶Ｐ１２５ＦＡＫ在肺癌细胞上的表达情况。结果：β１整合素抗体可明显抑制非小细胞肺癌的粘附作用，β１整合素在１０种人肺癌细胞中均有表达，而Ｐ１２５ＦＡＫ在小细胞肺癌中的表达则明显减少。结论：β１整合素在人非小细胞肺癌粘附到基底膜的过程中是不可缺少的，而Ｐ１２５ＦＡＫ的缺乏则可能导致小细胞肺癌的悬浮生长。

Ten human lung cancer cell lines have been used to detect β 1 integrin and focal adhesion kinase (P 125FAK ) as well as cell attachment assay.Anti β 1 integrin antibody inhibits adhesion of non small cell lung cancer cell lines.β 1 integrin expressed on all ten lung cancer cell lines.P 125FAK had a normal expression on non small cell lung cancer cell lines,but a less expression on small cell lung cancer cell lines.This interesting expression may be related to their different growth and invasion characters.