不同抗生素对罗汉果遗传转化受体系统的影响

Effects of Different Antibiotics on Receptor System of Siraitia grosvenirii Genetic Transformation

本研究探讨了抗生素潮霉素(Hyg)、头孢霉素(Cef)、氨苄青霉素(Ap)和羧苄青霉素(Carb)对罗汉果遗传转化受体系统的影响,确立了其在罗汉果转基因过程中的剂量方案。研究结果表明:氨苄青霉素、羧苄青霉素对罗汉果子叶离体培养的毒性比头孢霉素大;以头孢霉素抑菌效果最佳,250mg/L已经能完全抑制农杆菌的生长;用潮霉素(Hyg)罗汉果转基因植株进行抗性筛选,结果表明选择剂Hyg在不定芽和不定根抗性筛选中的临界浓度分别为25mg/L和10mg/L。

In this research,we approached the effects of different antibiotics,hygromycin（Hyg）,cefotaxime（Cef）,ampicillin（Ap） and carbenicillin（Carb） on receptor system of Siraitia grosvenirii genetic transformation,and established the dosage regimen of these antibiotics in the process of Siraitia grosvenirii transgenes.The result showed that higher toxicity of ampicillin and carbenicillin to Siraitia grosvenirii explants was observed than that of cefotaxime.Cefotaxime is optimal antibiotic on shoot frequency of Siraitia grosvenirii cotyledon,and 250 mg/L cefotaxime was completelys equal to suppress proliferation of A.tumefaciens.Different concentration of hygromycin（Hyg） were used for resistance screening of Siraitia grosvenirii transgenes plants,the results indicated that the suitable screening concentration of adventitious bud was 25 mg/L,and the adventitious roots regeneration could be completely inhibited on medium containing 10 mg/L hygromycin.