非标记定量蛋白质组学在烟草青枯菌致病性研究中的应用

The Application of Laber-free Quantification Proteomics in Tobacco Ralstonia solanacearum Pathogenicity Research

烟草青枯病是由茄科劳尔氏菌(Ralstonia solanacearum)引起的一种毁灭性的烟草种植灾害,可造成烟草的巨大减产。本实验室应用蛋白质组学中的Shotgun技术、非标记质谱定量方法和MA plot等对致病和非致病青枯菌蛋白质进行了生物信息学分析,从蛋白质组水平研究烟草青枯菌的蛋白质结构和功能,为了解其致病机理和防治提供新的方向。本文采用Shotgun技术在非致病性和致病性菌株中分别找到1628和1346个蛋白质,其中287个为非致病性菌株中特有,15个为致病性菌株中特有。对于两种菌株共有的1341个蛋白质用MA plot方法分析得到了163个显著性量变差异,并采用GO富集分析,显示它们主要定位于细胞外(intracellular part)或与糖代谢相关,为进一步揭示青枯菌侵染烟草的生物学机制提供了有力的支持。

Ralstonia solanacearum（synonym pseudomonas solanacearum） is a widely spread soli-borne plant pathogen which can cause＂bacterial wilt＂,a major bacterial plant diseases that greatly impact the production of tobacco.In this experiment,we discuss the structure and function of Ralstonia solanacearum on protein level by employing Shotgun method,label-free mass spectrometry relative quantitative strategy and MA-Plot,that make a new basis for its pathogenicity and bio-protection research.As our result,1 628 and 1 346 proteins were identified by the shotgun method in avirulent and virulent strains,respectively.287 proteins are unique in avirulent stains and 15 proteins in virulent strains.For the common 1 341 proteins in both strains,we finally identified 163 differential expressed proteins through MA-Plot analysis.The GO enrichment analysis shows that most of them are located in the intracellular part or take part in the sugar metabolism process.It provides strong support for further study of the mechanism of strain invasion on tobacco.