慢性阻滞一氧化氮合成致孕鼠妊娠高血压综合征样变化的研究

Chronic blockade of nitric oxide synthesis in pregnant rats produces signs similar to those of hypertension of pregnancy

目的：探讨在妊娠鼠中慢性抑制ＮＯ的合成，产生妊高征样症状。方法：妊娠鼠随机分为３组，于妊娠１４ｄ腹腔内连续注射生理盐水１ｍｌ（对照组）、亚硝基左旋精氨酸甲酯（Ｌ－ＮＡＭＥ）每只鼠每天３０ｍｇ（用药组）、Ｌ－ＮＡＭＥ每只鼠每天６０ｍｇ（双倍用药组）。记录收缩压、尿蛋白、血小板、胎仔重量及大小和胎盘重量及大小、畸形率及死亡率。结果：收缩压在用药组及双倍用药组与对照组比较有明显增加，尿蛋白在用药后与用药前比较明显增加，血小板用药组与对照组比较明显减少，胎仔的重量、大小及胎盘重量及大小，用药组及双倍用药组与对照组比较明显减少且畸形率和死亡率增加，有剂量依赖性。结论：慢性阻滞ＮＯ合成，可引起高血压、胎儿发育迟缓，出现尿蛋白、血小板减少。可提供与妊高征相似的动物模型。

Objective:Our purpose was to test the hypothesis that chronic inhibition of nitric oxide synthesis in pregnant rats can produce hypertension of pregnancy like syndrome.Methods:Pregnant rats were divided into three groups.On day 14 of gestation we continously infused them intraperitoneally with salt solution (control group),with L Nitro arginine methylester (L NAME) 30 mg/per rat/day (medicine group),with L NAME 60 mg/per rat/day (double medicine group).Systolic blood pressure,urinary albumin,platelet count,weight and size of newborn pups and placenta and several other parameters were determined.Results:Systolic blood pressures in medicine group and double medicine group were significantly increased,compared with control group.Urinary albumin was significantly increased after taking medicine than before taking medicine.But platelet count was significantly decreased in medicine group than in control group.This treatment also caused a substantial decrease in the weight and size of pups and placenta and increase in deformity and mortality. These effects were dose dependent.Conclusion:Chronic inhibition of nitric oxide synthsis in pregnant rats leads to sustained hypertension,fetal growth retardation,proteinuria,and thrombocytopenia and a simple animal model of hypertension of pregnancy can be provided.