摘要
目的 建立大鼠大脑中动脉局灶性缺血再灌注及缺血耐受模型,探讨该模型的应用价值.方法 将88只Wistar大鼠随机分成假手术组(8只)、假手术+再缺血组(NIP,40只)、缺血预处理+再缺血组(IP,40只),后两组再随机分成5个亚组.线栓法阻塞大脑中动脉(MCA)完成局灶性缺血预处理(缺血预处理10min),分别在缺血预处理后1,3,7,14,21d进行再次缺血2h再灌注22h后处死.模型建立后通过神经功能评分、TTC染色测定脑梗死体积及细胞凋亡等对模型进行评估.结果 ①组间比较:与NIP组相比,IP组中的1,3,7d亚组神经功能评分及脑梗死体积明显降低(P<0.05或P<0.01);IP组中的1,3,7,14d亚组凋亡细胞计数明显减少(P<0.05).②组内比较:IP组各亚组间神经功能评分差异无显著性;IP组1,3,7d亚组梗死体积较14,21d亚组明显减小,其中以3d亚组梗死体积减小最为明显(P<0.05);IP组以3,7d亚组平均凋亡细胞计数较其他亚组明显减少 (P<0.05).结论 线栓法制备脑缺血耐受模型切实可行,具有一定的应用价值.
Objective To establish and evaluate cerebral ischemic tolerance model induced by focal ischemic preconditioning in rats. Methods Eighty-eight male Wistar rats were randomly divided into three groups : sham operation group( n = 8 ) , non-ischemic preconditioning( NIP, n = 40 ) group, and ischemic preconditioning ( IP, n = 40 ) group. For isehemic preconditioning, the rats were given middle cerebral artery occlusion(MCAO) for 10 minutes. In the IP group,rat models of pre-ischemia-reperfusion-ischemia-reperfusion were established by MCAO using the twice suture method. In the NIP group, pre-ischemia was replaced by sham operation. Subsequently, the IP and NIP group were equally divided into five subgroups, that was 1- ,3-,7-, 14-, and 21-day subgroup. The rats in the sham operation group received the sham surgery two times. The models were evaluated through the following index : neurologic deficit scores, infarct volume and nerve cell apoptosis. Results (1)Intergroup comparison:compared with the NIP group,neurologic deficit scores and infarct volume significantly decreased in the 1,3,7d subgroups of IP group( P 〈0.05 ;P 〈0.01 ). The number of apoptotic cells significantly decreased in the 1,3,7 and 14d subgroups of IP group ( P 〈 0.05 ). (2)In the IP group, there was no significant difference in neurologie deficit scores among subgroups, infarct volume significantly decreased in the 1,3 ,Td subgroups( P 〈0.05) , the number of apoptotic cells significantly decreased in the 3 ,Td subgroups( P 〈 0. 05). Conclusion The results showed that 10-minute pre-ischemia induces cerebral ischemic tolerance to a subsequent severe ischemic insult, the effect of which is still present after 7 days of reperfusion. It is practicable for preparation of a rat model of ischemic tolerance induced by focal ischemic preconditioning.
出处
《潍坊医学院学报》
2010年第2期126-129,共4页
Acta Academiae Medicinae Weifang
关键词
脑缺血
缺血耐受
缺血预处理
凋亡
Brain ischemia
Ischernic tolerance
Ischemic preconditioning
Apoptosis