按摩对骨骼肌损伤修复过程中bFGF表达的影响

Massage the bFGF expression to affect to the skeletal muscle damage repair process in

目的：本研究旨在观察大鼠急性拉伤后腓肠肌bFGF表达的时间规律，以及按摩对骨骼肌损伤修复过程中bFGF表达的影响；探讨按摩治疗骨骼肌损伤的作用机理。方法：采用自制拉伤装置造成大鼠双后肢腓肠肌急性拉伤模型，损伤后第2d进行按摩治疗，并采用免疫组化法和图像分析系统观察大鼠腓肠肌自然修复过程中及按摩后bFGF表达的变化。结果：7d时大鼠腓肠肌bFGF平均光密度值达本实验最大值；按摩治疗3d组大鼠腓肠肌bFGF表达水平较同期自然修复组明显降低，有极显著性差异；按摩治疗5d组与同期自然修复组比较显著降低，有极显著性差异。结论：急性拉伤后7d内大鼠腓肠肌bFGF阳性表达呈现上升趋势；推测按摩通过促进bFGF在损伤修复过程中对靶细胞的作用，有效缩短了修复过程，此可能为按摩促进骨骼肌损伤修复的机制之一。

Objective： After this research is for the purpose of observing the big mouse acute pulling injury fei the intestines myo -- bFGF expression time rule,as well as massage to skeletal muscle damage repair process in bFGF expression influence; Discussion massage treatment skeletal muscle damage action mechanism. Methods： Uses the self--made pulling injury installment to create the big mouse double hind legs fei intestines myo-- acute pulling injury model,after the damage 2d carries on the massage treatment,af- ter and uses the immunity group reduction and the image analysis system observes in the big mouse fei intestines myo-- nature re- pair process and the massage the bFGF expression change. Results：When 7d the big mouse fei intestines myo-- bFGF average light density value reaches this experimental maximum value; The massage treats 3d the group big mouse fei intestines myo-- bFGF ex- pression level to compare the same time nature repair group to reduce obviously, has the extremely significance difference; The mas- sage treats the 5d group and the same time nature .repair group quite is remarkable reduces, has the extremely significance difference. Conclusion： After the acute pulling injury in 7d the big mouse fei intestines myo-- hFGF masculine gender expression presents the trend of escalation; The extrapolation massage through promotes bFGF in the damage repair process to the target cell function,reduced the repair process effectively, this possibly one of for massage promotion skeletal muscle damage repair mechanisms.