肺缺血再灌注损伤后TNF_α、IL-6和IL-8的变化及其意义

The Changes of TNF_α,IL-6 and IL-8 in Lung and Plasma in Rats With Lung Ischemia-reperfusion Injury and Their Singificances

为探讨炎性细胞因子在肺缺血再灌注损伤发病机制中的作用，采用大鼠在体温缺血再灌注模型，动态测定了肺组织和血浆肿瘤坏死因子α（ＴＮＦ_α）、白介素-６（ＩＬ－６）和白介素－８（ＩＬ－８）含量的变化。结果表明，肺缺血再灌注损伤早期肺组织ＴＮＦ_α、ＩＬ－６、ＩＬ－８相继释放增加，上述因子的血浆变化滞后于肺组织变化。提示炎性细胞因子参与了肺缺血再灌注损伤的发生和发展过程。

To investigate the role of inflammatory cytokines in the pathogenesis of lung ischemia-reperfusion injury , the rat ischemia model with occlusion of left hilus for 45-minute was set up and the changes of levels of TNF_α,IL-6 and IL-8 in lung tissues and plasma were dynamically observed during lung ischenia-reperfusion. The results showed that the releases of TNF_α,IL-6 and IL-8 in lung tissues were successively increased in the early stage of lung damage and the alterations of above-mentioned cytokines in plasma lagged those in lung tissues. It is concluded that inflammatory cytokines could participated in the onset and development of lung ischemia-reperfusion injury.