傅里叶变换红外光谱技术在死亡时间推断中的运用

Review on Estimation of Postmortem Interval Using FTIR Spectroscopy

死亡时间(postmortem interval,PMI)推断是命案现场首先要解决的重要问题之一,因此法医学者运用了大量的技术和统计学方法试图精确推断PMI。由于PMI推断受到外部、内部、死亡前和死亡后等多种因素影响,既往多种方法均存在局限性。傅里叶变换红外(Fourier transform infrared,FTIR)光谱技术已应用于蛋白质、核酸、碳水化合物的纯品物质研究上,近年来也被广泛用于研究复杂的细胞和组织。功能强大的计算机软件可以对光谱变换、平滑、基线校正、归一化等进行处理,使FTIR光谱仪对样本的定量研究成为可能,并从分析化学领域推广到生物学和临床医学研究。本文综述了FTIR光谱原理及其在生物医学中的应用,并着重阐述死后尸体组织的FTIR光谱学变化及在PMI推断中的应用价值。

Estimation of the postmortem interval（PMI） is a practical task in daily forensic casework.Researches on PMI is an important practical project in forensic field.Estimation of the time since death is influenced by internal and external,antemortem and postmortem factors,thus the old methods have limitations.Fourier transform infrared（FTIR） spectroscopy has been applied to study the pure protein,nucleic acid and carbohydrate and to detect the changes in complex cells and tissues.At present because the powerful software has could be used to achieve the spectrum transformation,smoothing,baseline correction and normalization,it is possible to analyze the samples quantitatively with the FTIR which has been applied in the biology and clinical medicine.This paper has reviewed the mechanism of FTIR and its application in biomedicine.The postmortem FTIR spectral changes were also discussed,which showed its potential for estimating PMI.