细粒棘球蚴中国大陆株铁蛋白生物信息学的初步分析及其结构和功能预测

Bioinformatics Analysis for the Structure and Function of Ferritin Gene from Echinococcus Granulosus(Chinese Mainland Strain)

目的应用生物信息学分析软件预测细粒棘球蚴中国大陆株铁蛋白(Eg.ferritin)氨基酸序列的结构与功能。方法利用DNAman、NCBI/BLAST公共数据库对目的基因的同源性进行比较分析。应用DNAstar、Bi-osun等生物分析软件对铁蛋白的二级结构、抗原表位进行预测分析,应用SWISSMODEL对蛋白的三维结构进行模拟。结果检索Eg.ferritin氨基酸序列在其功能区的130AA内同源性为97.7%。不同生物间的同源性平均达40.79%。生物软件预测:Eg.ferritin的分子量约16.7 kD,含非极性氨基酸68个,预测其抗原表位肽段为7N-12E,36H-43V,55S-62H,69Q-76R,82A-89E,102I-107E,117A-124S,129L-136T。结论 Eg.ferritin的结构和功能及其抗原表位的预测对选取有价值的抗原肽段提供了依据。

Objective To predict the structure and function of Eg.ferritin using bioinformatics method.MethodsHomology of ferritin gene was analyzed Using DNAman and NCBI/BLAST database and second structure and antigen peptide of Eg.ferritin and imitate 3D structure of Eg.ferritin was predicted using DNAstar,Biosun et.al.biological softwares to.ResultsDeduced ferritin amino acid sequence homology was 97.7% in the 130AA and average homology of different species was 40.79% with the published ferritin gene.And it had 68 nonpolar amino acids and had 9 antigen peptide such as 7N-12E,36H-43V,55S-62H,69Q-76R,82A-89E,102I-107E,117A-124S,129L-136T.ConclusionsUsing softwares to predict Eg.ferritin structure and antigen peptide can provide some theoretical bases for selecting some valuable antigen peptides.