宁夏回族甲硫氨酸合酶基因A2756G多态性研究

Methionine Synthase Gene A2756G Polymorphism in Hui Population of Ningxia,China

目的了解宁夏回族甲硫氨酸合酶(MS)基因A2756G的多态性的分布特点。方法通过聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术分析200例宁夏回族MS基因A2756G基因型。结果宁夏回族人群中AA、AG、GG基因型频率分别为0.8100、0.1750、0.0150,G突变基因频率为0.1025。与白种人群中的分布差异有统计学意义(P<0.05)。结论甲硫氨酸合酶基因A2756G基因型在不同种族间的分布存在明显的差异。

Objective To explore the genotype and allele distribution of methionine synthase gene A2756G in Hui Population.MethodsGenotypes were determined by using polymerase chain reaction-restrict fragment length polymorphism（PCR-RFLP）.ResultsMethionine synthase genetype frequency in Ningxia Hui was AA0.8100,AG0.1750,GG0.0150.The frequency of G was 0.1025,which was significantly different from that of white people（P〈0.05）.ConclusionThe MS genes A2756G distribution in Hui Nationality Population is different from those in other areas.