固相萃取-高效液相色谱法测定水源水中痕量双酚A

Determination of Bisphenol A in Source Water by Solid Phase Extraction-High Performance Liquid Chromatography

利用固相萃取-高效液相色谱建立了检测水源水中痕量双酚A的方法。同时，考察了C18小柱的最佳洗脱条件。此方法的色谱分析条件是：流动相为0．01moL／L的乙酸铵缓冲液（pH=4）与乙腈之比等于55-45（体积比），色谱柱温度为25℃，流速为1mL／min，检测波长为278nm。该方法的线性范围为50～1000μg／L，检出限（S／N=3）为0．5μg／L，不同加标水平的双酚A回收率为95．07％-98．53％。最佳固相萃取条件为：水样pH=3、用体积分数为75％的乙腈溶液作为洗脱剂、过样流速为5mL／min。用此方法测定实际水源水，结果准确、操作简便。

It used the SPE - HPLC to determinate bisphenol A in source water. The best elution condition of C18 column was described. Chromatographic conditions included： 0.01 mol/L mobile phase of ammonium acetate buffer（ pH - 4） ： acetonitrile = 55 ： 45 （ volume ratio）, the column temperature was 25℃, current velocity was 1.0 mL/min and the detection wave length was 278 nm. The linearity was in the range from 50 to 1 000 p,g/L,and detection limit（ S/N =3） was 0.5 ug/L. Recoveries for bisphenol A at different spiked levels were ranged from 95.07% to 98.53%. The best condition of SPE included ： pH of samples was 3, using aeetonitrile （75 %, volume ratio） as eluant, flow rate of samples was 5 mL/min. This method was simple, handled easily and accurate for the determination of bisphenol A.