大麻素WIN55,212-2对体外培养牛眼小梁细胞前列腺素E_2表达的影响

Effect of WIN55,212-2 on the Expression of PGE_2 in Cultured Bovine Trabecular Meshwork Cells

目的研究大麻素WIN55,212-2对体外培养的牛眼小梁细胞前列腺素E2(PGE2)表达的影响,从而探讨其降眼压机制。方法采用组织块培养法对牛眼小梁细胞进行原代培养,取传3代的细胞运用酶联免疫吸附(ELISA)法测定不同浓度大麻素WIN55,212-2作用后细胞上清液中PGE2水平的变化;运用RT-PCR方法检测不同浓度大麻素WIN55,212-2作用后PGE2mRNA在小梁细胞内的表达水平。结果细胞上清液中的PGE2水平与细胞内PGE2mRNA水平随大麻素浓度变化呈剂量依赖性升高。结论一定剂量的大麻素可以促进小梁细胞分泌PGE2,从而达到降低眼压的目的 。

Objective To study the effect of WIN55,212-2 on the expression of POE2 in cultured bovine trabecular meshwork cells （TMCs） and discuss its mechanism of reducing the intraocular pressure. Methods TMCs were obtained through the cultured tissue, bovine TMCs were prmiarily cultured and subcultured. The third generation bovine TMCs were incubated with different dosages of WIN55,212-2 and the supematant was collected. The concentration of PGE2 in the medium was measured by ELISA method. PGE2mRNA in TMCs was analyzed by RT-PCR. Result PGE2 in the supernatant and POE2mRNA in TMCs increased depending on the dosages of WIN55,212-2. Conclusion Certain concentration of WIN55,212-2 may promote POE2 secretion of TMCs, so as to reduce the intraocular pressure.