肺气虚证模型大鼠肾组织钠离子转运相关蛋白表达变化研究

Study on Expression of ENaC and rBSCl of Kidney Tissue in Lung-Qi Deficiency Model Rats

目的检测肺气虚大鼠肾脏组织与钠离子转运相关蛋白表达,为中医学肺肾相关理论提供实验依据。方法复制肺气虚大鼠模型,应用免疫组化方法检测肾小管上皮性Na+通道(ENaC)、Na+-K+-2Cl-转运体(rBSCl)蛋白表达,放免方法测定血浆与肺组织中醛固酮(ALD)、心房钠尿素(ANP)含量。结果与对照组比较,模型组大鼠肾小管ENaC、rBSCl蛋白表达明显上调,同时血浆与肺组织中ALD水平升高而ANP水平下降。结论肺组织通过释放ALD和ANP调节肾小管上皮细胞ENaC、rBSCl蛋白表达,从而调节肾小管对Na+与水的重吸收而影响机体水液代谢过程。

Objective To study the relationship of lung and kidney in TCM by detecting the protein expression related to Na^＋ transportation in the kidney tissue of Lung-Qi deficiency model rats. Methods Lung-Qi deficiency model of rats were copied. Immunohistochemistry was applied to measure the expressions of ENaC and rBSCI in renal tubular epithelium. RIA was used to detect ALD and ANP levels in plasma and lung tissue. Results Compared with the control group, expressions of ENaC and rBSCI of renal tubule were upregulated markedly, ALD level increased and ANP level decreased obviously in the model group. Conclusion Lung can regulate the expressions of ENaC and rBSCI in the renal tubule by secreting ALD and ANP, which can regulate Na^＋ and water reabsorption in the renal tubule and then influence the water metabolism of the body.