常春油麻藤中刺芒柄花素的提取工艺研究

Study on Extraction and Separation of Formononetin in Mucuna Sempervirens Hemsl

目的建立常春油麻藤中刺芒柄花素的提取方法。方法采用正交试验对刺芒柄花素的提取条件进行相关研究。结果甲醇作为提取溶剂,料液比1:40(g:mL),85℃回流提取3次,每次1.5h,常春油麻藤中刺芒柄花素含量为0.03812%。结论本研究优化确定了常春油麻藤中刺芒柄花素的提取方法 。

Objective To establish the method of extraction and separation of formononetin in Mucuna sempervirens Hemsl.. Methods The extraction and separation conditions of formononetin was studied by orthogonal test. Results Solid-liquid ratio was 1 ： 40 （g ： mL）, with methanol refluxing and extracting for 3 times at 85 ℃, 1.5 h once, the content of formononetin was 0.038 12%. Conclusion The optimal parameters for extraction and separation condition of formononetin in Mucuna sempervirerts Hemsl. was established.