胸导管引流对大鼠重症急性胰腺炎诱发肝脏损伤的保护作用

The protective effect of the thoracic duct drainage on liver injury due to severe acute pancreatitis in rats

目的观察胸导管淋巴引流在重症急性胰腺炎(SAP)诱发肝损伤的作用,为指导临床治疗提供实验依据。方法大鼠随机分为假手术组(S组)、重症胰腺炎模型组(SAP组)、胸导管引流组(TD组);每组又分为2、6、12h3个时间点。采用经胰胆管逆行注射5%牛磺胆酸钠法制备大鼠急性胰腺炎模型,检测大鼠腹水情况、血浆中肝功能指标(ALT、AST、TBIL)、TNF-α和IL-10的含量及胰腺和肝脏的病理变化。结果与SAP组比较,TD组腹水量明显减少,颜色变浅;ALT、AST、TBIL的含量明显下降(P<0.05或0.01);TNF-α和IL-10的含量明显下降(P<0.05或<0.01);显著改善胰腺和肝脏的病理损伤。结论胸导管引流可减轻重症急性胰腺炎合并的肝脏损伤。

Objective To investigate the protective effect of thoracic duct drainage on liver injury due to severe acute pancreatitis （SAP） in rats. Methods The rats were randomly divided into sham operation group, severe acute pancreatitis model group, thoracic duct fistula group, then every group was subdivided into 3 small groups according to time points of 2h, 6h, 12h. The SAP rat models were established via retrograde injection of 5% sodium taurocholate to the pancreatic duct. The ascites amount, the serum levels of ALT, AST, TBIL, TNF-a, IL-10 were detected. The changes of pancreas and liver tissue were observed. Results As compared with those in model group, the amount of ascites, the levels of ALT, AST, TBIL, TNF-a, IL-10 were decreased, and the psthological changes of pancreas and liver tissue were significantly improved in thoracic duct fistula group. Conclusion The neck fistula intervention can decrease hepatic injury in SAP rats.