赤潮铜绿微囊藻rDNA基因间隔区的序列分析以及与淡水微囊藻的比较

SEQUENCE ANALYSIS AND COMPARISON OF rDNA ISR REGIONS OF Microcystis SPECIES FROM RED TIDE AND NORMAL REGIONS

采用ＰＣＲ及序列测定的方法，对在厦门西港海域采集的赤潮铜绿微囊藻１６Ｓ和 ２３５ｒＤＮＡ基因间隔区Ｉｎｔｅｒｇｅｎｉｃ Ｓｐａｃｅｒ Ｒｅｇｉｏｎ （ＩＳＲ）区进行了序列测定和分析，并通过与两 种淡水微囊藻的比较，找出了其特征性核苷酸作为专一性分子探针设计的靶序列，为该藻 种以及微囊藻属的快速鉴定及系统学研究提供了分子基础。

The rDNA Intergenic Spacer Region (ISR) was sequenced and analyzed from a 'Red Tide' species Mlerocystis aeruginoea collected from Xiamen. The spacer sequences was 360 bp in lengh exclusive of 16S and 23S rDNA coding regions,containing a lle tRNA gene. Based on the analyses of the obtained sequences and other two sequences from fresh water strains, the evolutionary affiliation between species was discussed.