足月妊娠羊水过少血栓素、前列环素及胎盘组织血栓素受体表达的研究

A study of thromboxane and prostacyclin and thromboxane receptor expression in placenta of oligohydramnios.

目的:检测羊水过少孕妇静脉血中血栓素B2(TXB2)、6-酮前列腺素(6-keto-PGF1α)及胎盘组织中血栓素受体(TP)的表达,探讨其与羊水过少发病的关系。方法:ELISA法检测孕妇静脉血中TXB2、6-keto-PGF1α水平,Western blot检测胎盘组织TP含量,免疫组织化学法检测TP的表达及分布。结果:羊水过少孕妇血浆中TXB2、6-keto-PGF1α水平无明显变化,但胎盘组织TP蛋白表达增加,主要于滋养细胞层、绒毛间质表达。结论:胎盘滋养细胞层、绒毛间质TP表达增加,可能参与了羊水过少的发生。

Objective：To investgate the concentration of thromboxane and prostagladin in plasma and the expression of thromboxane receptor in placenta of oligohydramnios,and to prove its relation to oligohydramnios.Methods：Thromboxane A and prostagladin were examined by their stable metabolites of TXB2 and 6-keto-PGF1α by ELISA,thromboxane receptor was examined by Western blot and immunohistochemistry.Results：TXB2 and 6-keto-PGF1α did not show any change in plasma,but intense staining of TP receptor was seen in syncytiotrophoblast layer and stromal cells in placental tissue sections of oligohydramnios.The extensive expression of thromboxane receptor in placenta leaded villus core vessels to contract and the perfusion of placenta to decrease,thus oligohydramnios happened.Conclusion：The intense staining of thromboxane receptor in syncytiotrophoblast layer and in stromal cells in placneta perhaps plays a role in oligohydramnios.