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钙调蛋白在C型Niemann-Pick病小鼠Purkinje细胞的表达 被引量：2

The Expression of Calcium-related Proteins in Purkinje Cells of Niemann-Pick Type C Mice
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摘要 目的:探讨钙调蛋白在C型Niemann-Pick病(NPC)小鼠Purkinje细胞的表达。方法:采用免疫组织化学和Westen Blot技术检测3种钙调蛋白Calbindin D28k、IP3R1、SERCA2b在4、6、8周龄NPC小鼠小脑部的表达,以同周龄WT(野生型)小鼠作为对照组。结果:CalbindinD28k和IP3R1在6、8周龄时表达较WT小鼠显著下降,在4周龄时无明显减少,SERCA2b在8周龄时表达较WT小鼠显著下降。结论:3种钙调蛋白表达的下降可能导致钙失衡,参与神经元变性。 Objective：To investigate the expression of calcium-related proteins in Purkinje cells of Niemann-Pick Type C（NPC） mice.Methods：Immunohitochemistry and Western Blot were used to detect the expressions of calcium-related proteins including Calbindin-D28k,IP3R1,and SERCA2b in the cerebellum of 4,6,8 week-old NPC and wide-type（WT） mice.Results：When compared with WT,the expressions of Calbindin-D28k and IP3R1 were obviously down-regulated in the 6 and 8 week-old NPC mice whereas there was no significant decrease in the 4 week-old mice.SERCA2b was also down-regulated in 8 week-old mice.Conclusion：The down-regulated expressions of the calmodulin may lead to abnormal calcium dynamics and in turn be implicated in the degeneration of Purkinje cells.
作者 马丽丽 余姗姗 王雪贞 卜碧涛
机构地区 华中科技大学同济医学院附属同济医院神经科
出处 《神经损伤与功能重建》 2010年第3期157-162,共6页 Neural Injury and Functional Reconstruction
基金 国家自然科学基金项目(30670735)
关键词 NPC小鼠 Purkinje细胞变性 CalbindinD28k IP3R1 SERCA2b Niemann-Pick Type C（NPC） mouse degeneration of Purkinje cells Calbindin-D28k IP3R1 SERCA2b
分类号 R741 [医药卫生—神经病学与精神病学] R741.02 [医药卫生—神经病学与精神病学]
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参考文献20

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二级参考文献11

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共引文献6

同被引文献32

  • 1吕刚,韩笑,张秀娟,徐卫平,杜培革.半胱氨酰白三烯受体的研究进展[J].吉林医药学院学报,2008,29(3):164-167. 被引量：4
  • 2张向鑫,李庆浩,李清.P-选择素与心肌缺血再灌注损伤[J].社区医学杂志,2004,2(3):26-28. 被引量：6
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二级引证文献2

神经损伤与功能重建
2010年 第3期

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