降钙素基因相关肽对急性肝损伤小鼠肝脏微循环的干预

Interference of CGRP to hepatic microcirculation in acute liver injury

目的:研究降钙素基因相关肽(CGRP)对刀豆蛋白A(ConA)诱导的小鼠肝损伤模型肝脏微循环的影响。方法:昆明种小鼠60只,随机分为ConA损伤组、CGRP干预组和空白对照组(n=20),空白对照组注射生理盐水,ConA损伤组用ConA诱导建立小鼠急性肝损伤模型,CGRP干预组在用ConA诱导建模前体外注射CGRP,各组中10只于处理后用激光多普勒血流仪测肝脏的平均血流灌注量,另10只活体观察肝脏微循环流速,最后处死,全部HE染色观察肝脏病理变化。结果:CGRP干预组肝脏的平均血流灌注量和血液流速较ConA损伤组明显增加(P<0.01),病理学改变明显减轻。2组的血细胞浓度差异无统计学意义(P>0.05)。结论:CGRP能够通过改变肝脏组织的灌注影响急性肝损伤时肝脏微循环障碍的程度和病理改变。

Objective： To interview the role of CGRP to hepatic microcirculation in acute liver injury induced by coneanavalin A（Con A）. Methods： Sixty Kunming rats were divided into three groups randomly（n=20）, acute liver injury was established by injection with 20 mg/kg Con A through the tail vein. A saline control group was established by injection with saline. In CGRP-administered group, CGRP was given to rats 30 min before Con A injected. Ten mice in each group were used to observe the average liver blood flow volume and concentration by laser-doppler flow-instrument, and the other ten rats were observed the hepatic microcirculation velocity in vivo by an inverted microscope. Liver damage was assessed by histological evaluation after the rat had been killed. Results： Compared to the injury group, the average liver blood flow volume and velocity were significantly increased in CGRP-administered group, meanwhile, the pathological injury was markedly alleviated, whereas the blood concentration was almost the same. Conclusion： CGRP can decrease the dysfunction of hepatic microcirculation by means of improving the tissue perfusion, and alleviate the pathological damage during acute liver injury.