摘要
目的 探讨局部应用肝细胞生长因子（HGF）缓释微胶囊对诱导缺血心肌血管新生的作用.方法 30只新西兰大白兔随机分为对照组（Ⅰ组）,空白胶囊组（Ⅱ组）,HGF缓释微胶囊组（Ⅲ组）,每组10只.三组均开胸结扎冠状动脉前降支中点,Ⅱ组于左旋支、前降支交界区心外膜下埋藏空白微胶囊,Ⅲ组埋藏HGF缓释微胶囊各5只.术后6周,检测左心室功能指标,免疫组化测定心肌梗死边缘区微血管数.结果 与Ⅰ、Ⅱ组相比,Ⅲ组左心功能恢复好,微血管数目明显增多,分别为（101.28±19.50,105.28±18.28,161.28±15.85）.结论 HGF缓释微胶囊能促进兔缺血心肌血管新生,改善心功能.
Objective To evaluate the effect of slow-release microcapsules of HCF（ hepatocyte growth factor） on angiogenesis in infracted myocardium.Method Myocardial infarction was induced in 30 New Zealand rabbits by ligating the middle of left descending coronary artery. Group Ⅰ （ n = 10） was served as a control group, group Ⅱ （ n =10） as a blank microcapsule group, group Ⅲ （ n = 10） as experimental group with each microcapsule contains 1 μgHGF as HCF group. In group Ⅱ andⅢ, 5 blank microcapsules or FGF slow-release microcapsules were implanted into myocardium under epicedium between the left descending coronary artery and left circumflex branch. The heart function of each rabbit was evaluated with echocar-diography and cheterization, angiogenesis was evaluated by immunohistochemical technique 6 weeks later.Result As compared with group Ⅰ and Ⅱ , rabbits treated with HGF had higher microvessel counts （ P 〈 0. 01）, and LVFS and EF were significantly increased [ （101. 28±19. 50,105. 28 ±18. 28,161. 28 ±15. 85, P 〈0.01 ]. Conclusion Subepicardial implantation of HGF slow release microcapsule in the infracted rabbit model can enhance effective angiogenesis and improve left ventricular function.
出处
《中国医师杂志》
CAS
2010年第5期588-590,共3页
Journal of Chinese Physician
基金
江西省南昌市科技局资助项目,编号2006-10
