肾透明细胞癌中HIF-1α和Survivin表达及其相关性研究

Expression of HIF-1α and survivin and their relationship in renal clear cell carcinoma

目的 探讨肾透明细胞癌组织中缺氧诱导因子-1α（HIF-1α）和存活素（survivin）的表达、与Robson分期关系及两者的相互关系.方法 采用免疫组化法检测32例肾透明细胞癌及10例正常肾脏组织中HIF-1α和survivin的表达.结果 HIF-1α和survivin在肾透明细胞癌中的表达（分别为0.037±0.0182,0.093±0.0384）较正常组织（分别为0.008±0.0055,0.014±0.0059）明显升高（P〈0.05）;按Robson分期Ⅲ,Ⅳ期者（0.053±0.0179,0.137±0.0291）较Ⅰ,Ⅱ期者（0.029±0.0117,0.070 ±0.0154）明显增高（P〈0.05）;二者表达呈正相关（r=0.655,P〈0.05）.结论 肾透明细胞癌中HIF-1α和survivin表达增加且与分期相关;二者表达呈正相关（r=1.000.0.655,P〈0.05）.

Objective To investigate the expression of HIF-1α and survivin in renal clear cell carcinoma （RCC） and normal renal tissue （ NRT） and explore the correlation between HIF-1α and survivin expression. Method SABC immunohistochemical staining was performed to detect the expression in 32 cases of RCC and 10 cases of NRT.Results The level of positive HIF-1α immunostaining in RCC（0. 037 ±0. 0182,0. 093 ±0. 0384,respectively） was higher than NRT（0. 008 ±0.0055,0. 014 ±0. 0059, respectively） （ P〈0. 05）. The level of positive survivin immunostaining in RCC（0. 053 ±0.0179,0. 137 ±0.0291） was higher than NRT（ P〈0. 05）. The expression of HIF-1α and survivin had positive relationship with clinical stage in RCC（0. 029 ±0. 0117,0.070±0.0154）（ P 〈0.05）. The expression of HIF-la had relation with survivin in RCC（ r = 0. 655, P 〈 0. 05）.Conclusion The expression of HIF-1α was strongly positive correlated with survivin （ r = 1.000,0. 655, P 〈0. 05）.