树鼩5个生化位点与11个微卫星标记位点的初步研究

Elementary Study on Five Biochemistry Sites and Elever Microsatellites Markers Sites of Tree Shrews (Tupaia Belangeri Chinensis)

目的初步建立树鼩生化与微卫星标记检测法.方法参考近交系小鼠、大鼠生化遗传标记检测方法,对树鼩某些同工酶进行活性测定.根据树鼩特异DNA序列,合成引物,扩增树鼩基因组DNA.结果树鼩Es-1,Trf,Akp-1,Ce-2四个生化位点呈遗传多态性,而Es-3生化位点无遗传多态性;所选的11个微卫星DNA位点中,有9个具有高度多态性,2个微卫星DNA多态性较差.结论初步建立了树鼩生化及微卫星标记的方法,为深入研究树鼩的遗传背景积累了基础资料.

Objective To set up preliminarily the methods to detect biochemical genetic markers of tree shrews.Methods According to the methods of Laboratory animal-Genetic monitoring：methods for biochemical markers of inbred mice and rats,some isoenzyme activities of tree shrews were detected.The primers were synthesized on the basis of specific DNA fragment published before and the genomic DNA of tree shrews was amplified.Results Among the 6 biochemical loci,the locus Es-3 had only one allele,while the locus Es-1,Trf,Akp-1,and Ce-2 had polymorphism.Among the 11 microsatellites loci,9 polymorphic microsatellites loci and 2 non-polymorphic microsatellites loci were screened.Conclusion The detecting methods of biochemistry and microsatellites genetics markers of tree shrews have been established initially,and this may help to accumulate basic data for genetic background of tree shrews.