十溴联苯醚在单次暴露的孕和非孕SD大鼠血液内的吸收分布

Absorption and distribution of BDE-209 in the blood of pregnant and non-pregnant Sprague-Dawley rats exposed by single dose of decabromodiphenyl ether

五溴联苯醚、八溴联苯醚被禁以后,十溴联苯醚(BDE-209>97%)是现在唯一仍在全球范围内广泛使用的多溴联苯醚阻燃剂。BDE-209在环境介质和生物体,甚至人体的血液、母乳中普遍存在,而且BDE-209的环境含量呈上升趋势。在实验室条件下,BDE-209可以通过生物代谢,或光降解、热降解转换形成毒性更强的低溴代PBDEs、羟基甲氧基PBDEs、致癌物多溴二苯并二噁英/呋喃(PBDD/DFs)。所以BDE-209的环境行为及生物效应成为环境污染物领域研究的热点之一。目前,关于BDE-209对妊娠期生物体的暴露研究还是很少。研究了十溴联苯醚在单次暴露的孕和非孕SD大鼠血液内的吸收代谢。结果表明,BDE-209可以被孕和非孕SD大鼠吸收,孕和非孕SD大鼠血液中吸收的BDE-209都可以快速的排出。BDE209在孕和非孕SD大鼠体内存在脱溴代谢,主要的脱溴代谢物是3种nona-BDE(sBDE-206,-207,-208)和五种octa-BDEs(BDE-196,-197/204,-198/203),其中3种nona-BDEs是主要的代谢物,而BDE-207是nona-BDEs中含量最大的单体。BDE-209及其脱溴代谢物nona-BDEs的清除速率,在SD孕鼠的体内快于SD非孕鼠,而且在暴露实验的后期还存在统计意义的显著差异性。

Decabromodiphenyl ether（BDE-209）is currently the most widely used polybrominated diphenly ethers（PBDEs）flame retardant around the world since penta-BDE and octa-BDE were prohibited.BDE-209 has been ubiquitously found in abiotic and biotic compartments,and even in human blood and milk.Moreover,an upward trend of the content of BDE-209 in environment has been observed.Under laboratory conditions,BDE-209 can be transformed to more toxic lower brominated PBDEs,OH-PBDEs, Meo-PBDEs and carcinogenic polybrominated dibenzo-p-dioxins/furans（PBDD/Fs）by biological metabolism,photolysis and pyrolysis.,So,it has been a current research hotspot in the field of environmental science for the study on the environmental behavior and biological effects of BDE-209.However,little information is available for the exposure of pregnant animals to BDE-209 in the literatures.In this study the absorption and metabolism of BDE-209 in the blood of pregnant and non-pregnant Sprague-Dawley rats exposed by single dose of Decabromodiphenyl ether were studied.The results showed that BDE-209 can be absorbed by pregnant and non-pregnant Sprague-Dawley rats,and BDE-209 in blood can be eliminated quickly in pregnant and non-pregnant rats.Debrominated metabolism were observed in pregnant and non-pregnant rats and the major debrominated products were three nona-BDEs（BDE-206,207 and 208）and five octa-BDEs（BDE-196,197/204 and 198/203）.Among the debrominated metabolites of BDE-209 nona-BDEs were the predominant congeners and BDE-207 was the congener with the maximal concentration in blood. BDE-209 and its debrominated products nona-BDEs were eliminated faster in pregnant rats than in non-pregnant rats.Furthermore,a statistically significant difference of the elimination rate of BDE-209 and nona-BDEs was observed between pregnant and non-pregnant rats in the later phase of exposure.