毛橘红醇提物中柚皮苷、柚皮素在大鼠尿液和粪便中的代谢与排泄

UPLC-Q-TOF/MS analysis of naringin and naringenin and its metabolites in rat urine and feces after intragastric administration of alcohol extract of Exocarpium Citri Grandis

为分析毛橘红醇提物中柚皮苷、柚皮素在大鼠尿液和粪便中的代谢与排泄,本文给健康SD大鼠连续灌胃毛橘红醇提物3天,于第3天给药后,收集0～24h的粪便和0～4、4～8和8～24h的尿液,使用UPLC-Q-TOF/MS仪器与MetabolynxTM软件分析。结果显示,在大鼠含药尿液中存在柚皮苷及其6个代谢产物、柚皮素及其4个代谢产物;在含药粪便中存在柚皮苷及其3个代谢产物、柚皮素及其2个代谢产物。

To analyze naringin,naringenin and its metabolites in rat urine and feces after intragastric administration of alcohol extract of Exocarpium Citri Grandis,healthy SD rats were fed with alcohol extract of Exocarpium Citri Grandis for 3 days.On the last day,0-24 h feces and 0-4 h,4-8 h,8-24 h urine were collected and analyzed by UPLC-Q-TOF/MS.The post-acquisition data were processed using MetabolynxTM.The result is that naringin and its 6 metabolites,naringenin and its 4 metabolites were detected in the urine of rat.Meanwhile,naringin and its 3 metabolites,naringenin and its 2 metabolites were detected in the feces of rat.