摘要
Microcystins are a kind of cyclic hepatoxins produced by many species of cyanobacteria.Most previous work have been done on the toxic effects of microcystins on animals and plants.However,the reports about the effect of microcystins on microbial cells are very limited.In this work,the permeability of MC-RR on the cell outer membrane of Escherichia coli(E.coli) and Bacillus subtilis(B.subtilis) was discussed.The permeability effect of MC-RR on the cell outer membrane of E.coli and B.subtilis under different concentrations was demonstrated by a rapid and sustained reduction in the A675 values of lysozyme-treated cells.The decrease of the absorbance values showed a time-and dose-effect.The extravasations of protein and carbonhydrate increased with the increment of the treated-concentration of MC-RR.The results showed that MC-RR could increase the permeability of cell outer membranes of E.coli and B.subtilis.The synergistic effects of MC-RR and lysozyme on bacteria indicated that MC-RR might play an ecological role in bacteria in combination with other substances in some aquatic environments.
Microcystins are a kind of cyclic hepatoxins produced by many species of cyanobacteria. Most previous work have been done on the toxic effects of microcystins on animals and plants. However, the reports about the effect of microcystins on microbial cells are very limited. In this work, the permeability of MC-RR on the cell outer membrane of Escherichia coli （E. coli） and Bacillus subtilis （B. subtilis） was discussed. The permeability effect of MC-RR on the cell outer membrane of E. coli and B. subtilis under different concentrations was demonstrated by a rapid and sustained reduction in the A675 values of lysozyme-treated cells. The decrease of the absorbance values showed a time- and dose-effect. The extravasations of protein and carbonhydrate increased with the increment of the treated-concentration of MC-RR. The results showed that MC-RR could increase the permeability of cell outer membranes of E. coli and B. subtilis. The synergistic effects of MC-RR and lysozyme on bacteria indicated that MC-RR might play an ecological role in bacteria in combination with other substances in some aquatic environments.
基金
supported by the Knowledge Innovation Project of the Chinese Academy of Sciences (KZCX2-YW-Q07-04)
the Foundation of Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology, SOA (200713)
Open Research Fund Program of Shandong Key Laboratory for Eco-Environmental Science of Yellow River Delta, Binzhou China (2007KFJJ04)
