江西省龙牙百合种质资源遗传多样性研究

ISSR Analysis of Genetic Diversity of Lilium brownii var. viridulum Baker in Jiangxi Province

利用14个ISSR引物对江西省4个主要产地的100个龙牙百合(Lilium brownii var.viridulum Baker)个体进行了遗传多样性研究,共扩增出213条清晰谱带,其中多样性谱带165条。ISSR分析结果显示:(1)龙牙百合的遗传多样性水平较高,其居群平均水平为PPF=31.69%,Ne=1.198,I=0.168,Hpop=0.113;物种水平为PPF=77.46%,Ne=1.438,I=0.392,Hpop=0.260;(2)不同产地的种质间出现明显的遗传分化(AMOVA:ΦST=0.632,P<0.001);(3)聚类分析和主成分分析表明,来自同一产地的个体聚在一起,丰城市罗山居群(LS)与其他居群间的分化最大,藤田居群(TT)与苏溪居群(SX)的关系最近。

Lilium brownii var.viridulum Baker is an important medicinal plant species.Here we employed inter-simple sequence repeat （ISSR） markers to explore genetic diversity and patterns of genetic variation in 100 individuals from four cultivated populations in Jiangxi Province.Using 14 ISSR primers,213 discernible DNA fragments were scored with 165（77.5%）being polymorphic.High levels of genetic variation were revealed both at the population level（mean PPF = 31.69%,Ne = 1.198,I = 0.168,Hpop = 0.113）and the species level（PPF = 77.46%,Ne = 1.438,I = 0.392,Hpop = 0.260）.Strong genetic differentiation among populations（AMOVA：ΦST = 0.632,P 〈0.001）was also detected,which may result from artifical selection and long-term cultivation.The UPGMA cluster analysis and principal components analysis （PCA） revealed that individuals from the same population formed a distinct group,and LS population was clearly separated from other populations.On the basis of these observations,some suggestions for efficient management of the genetic resources of this important medicinal herb are proposed.