摘要
【目的】研究细菌磁小体对小鼠免疫应答的影响,为磁小体的体内应用提供参考依据。【方法】以卵清白蛋白(OVA)作为抗原分别与弗氏完全佐剂(阳性对照)、磁小体(BMP)悬液、PBS缓冲液(阴性对照)混合免疫小鼠。两周后检测血清中抗OVA特异性抗体及亚型、细胞因子表达水平以及淋巴细胞增殖能力。【结果】与阴性对照(PBS组)相比,BMP组在IL-2、IFN-γ和IL-4、IL-10的表达水平、淋巴细胞增殖能力、特异性抗体的产生没有明显差异。【结论】磁小体对小鼠免疫应答无显著的影响。
[Objective ]To study the influence of native bacterial magnetic particles on mouse immune response. [Methods]Ovalbumin was used as an antigen,mixed with complete Freund＇s adjuvant,bacterial magnetic particles （BMP） and phosphate buffer solution,to immunize BALB/C mouseAfter 14 days later,we detected the titers of the anti-ovalbumin （IgG） and subtype （IgG1,IgG2a）,the proliferation ability of T lymphocyte,and the expression of IL-2,IL-4,IL-10 and IFN-γ. [Results]Compared to the negative control group,the BMP group had the similar level in the titer of specific antibody,the proliferation ability of T lymphocyte and the expression of cytokines （IL-2,IL-4,IL-10 and IFN-γ）. [Conclusion]Native BMPs has no significantly influence on mouse immune response.
出处
《微生物学报》
CAS
CSCD
北大核心
2010年第6期817-821,共5页
Acta Microbiologica Sinica
基金
国家“863计划”(2007AA021805)~~
关键词
磁小体
免疫应答
细胞因子
MTT
bacterial magnetic particles
immune response
cytokines
MTT
