胰岛素对局灶脑缺血c-jun基因表达的影响

The effect of insulin on c jun gene expression in the rat brain with focal ischemia.

目的观察不同剂量胰岛素对高血压大鼠局灶缺血脑组织ｃｊｕｎ基因表达的影响。方法以肾血管性高血压大鼠（ＲＨＲ）复制大脑中动脉闭塞（ＭＣＡＯ）模型，采用原位杂交技术检测不同剂量胰岛素组和对照组ＭＣＡＯ后３ｈ脑组织ｃｊｕｎ基因的表达。结果与对照组相比，在缺血侧广泛的大脑皮层，低剂量胰岛素组ｃｊｕｎｍＲＡＮ有统计学意义的增加（Ｐ＜００５），而较高剂量胰岛素组ｃｊｕｎｍＲＮＡ增加更为显著（Ｐ＜００１）。结论胰岛素促进缺血脑组织ｃｊｕｎ基因表达可能是其神经保护作用机制之一，而且这种作用呈剂量依赖性。

Objective To investigate the effect of insulin on c jun gene expression following focal cerebral ischemia in hypertensive rats.Methods The renovascular hypertensive rats(RHR)were divided into 4 groups receiving either insulin only (Group A),insulin plus glucose(Group B and C,dose differently) or normal saline (Group D)immediately after middle cerebral artery occlusion (MCAO).The level of c jun mRNA was measured by situ hybridization at 3h after MCAO. Results Low or high dose of insulin all increased c jun expression in ipsilateral cortex following MCAO and c jun mRAN increased along with elevated insulin dose.Conclusions These results indicate that the effect of insulin on c jun expression may be one of the mechanisms of neuroprotection of insulin,which is dose dependent.