IL-13对正常人表皮成纤维细胞炎症及纤维化相关基因表达的影响

The Study of Inflammatory and Fibrosis-related Genes Expression on Interleukin 13 Treated Normal Human Dermal Fibroblasts

目的探讨IL-13对正常人表皮成纤维细胞(NHDF)炎症及纤维化相关基因的作用,从而进一步了解IL-13在皮肤纤维化过程中的病理机制。方法将不同浓度的IL-13(0.1ng/mL,1ng/mL,10ng/mL)加入到体外培养的NHDF,作用24h后,采用实时定量PCR(Real-TimePCR)方法 ,研究不同浓度IL-13刺激NHDF后CCL-2,IL-13ra1,IL-13ra2,CTGF和Pro-collagenI基因的表达情况。结果不同浓度的IL-13刺激NHDF24h后,CCL-2,IL-13ra2和Pro-collagenI基因表达显著增加(P<0.05),并呈剂量依赖关系;IL-13ra1和CTGF基因表达无明显改变(P>0.05)。结论 CCL-2,IL-13ra2和Pro-collagenI基因的高表达可能参与了IL-13在皮肤组织纤维化中的病理过程。

To investigate the effects of IL-13 on inflammatory and fbrosis-related genes expression in nordermal fibroblasts （NHDF）. Methods To add different concentrations of IL-13 （0.1 ng/mL, lng/mL, 10ng/mL） into the in-vitro-cultured NHDF, after 24-hours stimulation, extracted RNA, and applied the Real-Time PCR to investigate the genes expression of CCL-2, IL-13ral, IL-13ra2, CTGF and Pro- collagen I in IL-13 treated NHDF. Results After 24-hours IL-13 stimulation in NHDF, the genes express- ing of CCL-2, IL-13ra2 and Pro-collagen I were significantly and dose-dependently increased （ P 〈 0.05 ）, while the IL-13ral and CTGF genes expressing were not significantly increased （P 〉 0.05 ）. Conclusion Our results indicate that, CCL-2, IL-13ra2 and Pro-collagen I genes over expressing may involved in IL-13 pathological role in skin fibrosis .