成骨蛋白-1对髓核抽吸术后兔腰椎间盘影像学改变的影响

Radiographic effect of osteogenic protein-1 (OP-1) on lumbar disc after nucleus pulposus aspiration in rabbits

目的:观察成骨蛋白-1(osteogenic protein-1,OP-1)对髓核抽吸术后椎间盘影像学改变的影响。方法:32只日本大耳白兔随机分成2周、4周、8周、12周4组,所有兔子用21号针头行L3/4椎间盘后外侧穿刺,抽吸出部分髓核组织,用微量注射器注射OP-125μl作为实验组(OP-1组),行L1/2椎间盘穿刺抽吸部分髓核组织并注射25μl生理盐水作为对照组(Saline组),术后2周、4周、8周、12周时按照分组取8只兔子行腰椎侧位X线摄片检查,测量施术椎间隙高度并计算椎间盘高度指数(DHI),行正中矢状位MRI检查,观察T2加权信号强度改变。结果:X线检查结果表明,OP-1组及Saline组术后2周、4周、8周、12周椎间盘高度呈降低趋势,但OP-1组较Saline组高度降低缓慢,各时间点两组DHI差异显著(P<0.05);MRI检查结果表明,OP-1组椎间盘术后2周、4周、8周、12周的髓核信号强度均较同期的Saline组椎间盘髓核信号强度高,二者Thompson评分结果差异显著(P<0.05)。结论:OP-1能延缓后外侧纤维环穿刺髓核抽吸术后兔椎间盘X线高度的降低和MRI T2加权信号强度的减低。

Objective：To investigate the radiographic effect of osteogenic protein-1（OP-1）on lumbar disc after nucleus pulposus aspiration in rabbits.Method：32 Japanese rabbits were randomly divided into 2 weeks,4 weeks,8 weeks and 12 weeks group.All the rabbits′posterior annulus fibrosus of L3/4 disc were stabbed by 21-gauge hypodermic needle,then some nucleus pulposus tissues were aspirated out.25μl OP-1 were injected into test discs using micro-injection syringe as test group（OP-1 group）.While lumbar 1/2 disc were performed the procedure as lumbar 3/4 and 25μl saline were injected as blank control（Saline group）.2 weeks,4 weeks,8 weeks and12 weeks after operation,lateral X-ray was performed on 8 rabbits and the disc height as well as disc height index（DHI）were measured,and the T2 weighted signal intensity of each group was measured under MRI.Result：Disc height decreased gradually with time passing in both OP-1 group and without significant difference between the two groups,and at each time point,the difference of DHI between two groups was significant（P0.05）.MRI showed the nucleus pulposus signal intensity in OP-1group was higher than saline group at each time point after operation,which resulted in a significant Thompson score difference（P0.05）between 2 groups.Conclusion：OP-1 can delay the disc height loss and degrade MRI T2-weighted signal intensity after nucleus pulposus aspiration.