摘要
目的:检测胰岛素样生长因子结合蛋白7(IGFBP7)在皮肤黑素瘤组织和细胞系中的表达。方法:免疫组化法检测25例原发性皮肤黑素瘤、5例转移性皮肤黑素瘤、20例良性色素痣、10例正常皮肤组织和4株人黑素瘤细胞系中IGFBP7的表达。结果:IGFBP7在正常皮肤和良性色素痣细胞浆中的阳性表达率为85%,而在皮肤黑素瘤胞浆中的阳性表达率为16%和20%。除人黑素瘤细胞系MV3阳性表达IGFBP7外,其余细胞系均阴性表达。结论:IGFBP7在皮肤黑素瘤组织和细胞系中的低表达或功能缺失可能参与了皮肤黑素瘤的发病。
Objective: To detect the expression of insulin - like growth factor binding protein 7(IGFBP7) in ma- lignant melanoma and melanoma cell lines. Methods: The expression of IGFBP7 was examined by immunohistoche- mical technique in 25 cases of primary malignant melanoma, 5 eases of metastatic malignant melanoma, 20 cases of benign melanocytic naevi, 5 normal skin specimens and 4 malignant melanoma cell lines. Results: The expression of IGFBP7 was found in cytoplasm of 85 % of normal skin specimens and benign melanocytie nevi tissues, while the expression was only found in 16% of malignant melanoma tissues. Besides melanoma cell line MV3 which expressed IGFBP7, all other melanoma cell lines negatively expressed IGFBP7. Conclusions: The low expression or dysfunc- tion of IGFBP7 may be involved in the pathogenesis of melanoma.
出处
《中国麻风皮肤病杂志》
2010年第6期385-388,共4页
China Journal of Leprosy and Skin Diseases
基金
江苏省六大高峰人才基金资助(06-B-72)