Ganciclovir对转导HyTK基因人膀胱癌EJ细胞DNA含量的影响

Ganciclovir effect on the DNA content of human bladder carcinoma EJ cells transfected with HyTK gene

目的了解ＨｙＴＫ基因转移联合Ｇａｎｃｉｃｌｏｖｉｒ（ＧＣＶ）对人膀胱癌细胞的杀伤作用。方法采用５７０型粘附式细胞激光定量分析筛选仪测定ＧＣＶ作用下人膀胱癌细胞株ＥＪ细胞、转导ＨｙＴＫ基因的ＥＪ细胞（ＥＪ／ＴＫ）ＤＮＡ含量的变化。结果实验组ＥＪ／ＴＫ细胞ＤＮＡ平均荧光值１５９２０８±１８０７０，明显低于对照组２０４７１７±２２８４１（Ｐ＜０００１）；而ＥＪ细胞ＤＮＡ平均荧光值实验组为２２４６０６±２３４．６６，对照组为２２３２９７±２５２．０８，两组间差异无显著性（Ｐ＞０５）。结论ＧＣＶ作用能明显降低转导ＨｙＴＫ基因ＥＪ细胞（ＥＪ／ＴＫ）的ＤＮＡ含量。

Obejective To elucidate the ganciclovir effect on human bladder carcinoma cells. Methods The DNA fluorescence intensity of human bladder carcinoma cell line EJ cells and HyTK gene transduced EJ cells (EJ/TK) was measured by adherent cell analysis and sorting cytometer (ACAS570).In experimental group,EJ/TK cells and EJ cells were cultured respectively and treated with GCV of 100 μmol/L for 48h,while GCV was absent in controls.Cells were stained by propidium iodide (PI). Results The average DNA fluorescence value of EJ/TK7 cells was 1 592.08±180.70 and that of EJ cells was 2 246.06±234.66 in the experimental group;while the average DNA fluorescence value of EJ/TK cells was 2 047.17±228.41 and that of EJ cells was 2 232.97±252.08 in controls.There was no significant difference between experimental and control group of EJ cells ( P >0.5);Comparing with the control,thd average DNA fluorescence value of EJ/TK cells after exposure to GCV decreased significantly ( P <0.001). Conclusions GCV could obviously reduce the DNA content of HyTK gene transduced EJ cells and suggest HyTK gene transfer in conjunction with GCV have therapeutical effect on human bladder cancer.