治疗型关节炎护膝干预膝骨性关节炎患者滑液细胞因子的研究

OA-kneeguard Interferes with Cytokine of Synovial Fluid in the Patients with Osteoarthritis

目的探讨治疗型关节炎护膝(OA护膝)干预膝骨性关节炎的作用机制。方法选择膝骨性关节炎患者60例,随机分为OA护膝组和微波组各30例,分别采用OA护膝和微波治疗。分别测定两组关节滑液白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(INF-α)、基质金属蛋白酶-3(MMP-3)、透明质酸(HA)浓度。结果护膝组X线Ⅰ、Ⅱ、Ⅲ级患者治疗后IL-1β、IL-6、INF-α、MMP-3含量比治疗前降低,而HA含量升高,结果比较差异有显著统计学意义(P<0.01)。OA护膝组与微波组治疗后X线Ⅰ、Ⅱ级患者膝关节滑液IL-1β、IL-6、TNF-a、MMP-3、HA含量比较差异有统计学意义(P<0.01,P<0.05);两组X线Ⅲ级患者治疗后膝关节滑液IL-1β含量比较也差异有统计学意义(P<0.05),而IL-6、INF-α、MMP-3、HA含量比较差异无统计学意义(P>0.05)。结论 OA护膝可以有效调节细胞因子、MMP-3、HA的生物效应,从而延缓关节软骨的退变。

Objective To explore the interference of OA-kneeguard for the patient with knee osteoarthritis.Methods Sixty patients with knee OA were selected and randomly divided into OA-kneeguard groups and microwave group. The joint synovial fluid IL-1β （IL-1β）, intedeukin -6 （IL-6）, tumor necrosis faetor-α （TNF-ct）, matrix metalloproteinase -3 （MMP-3）, hyaluronie acid （HA） concentration were determined. Results IL-1β, IL-6, INF-α, MMP-3 content in X-ray kneepad Group I , II , III grade patients were lower than before treatment, while the HA content increased more than before treatment. The results were significantly different （P〈0.01,P〈0.05）. Knee OA group and microwave group X-ray after treatment I, II level in patients with knee joint synovial fluid IL-1β, IL-6, TNF-a, MMP-3, HA content had significant difference （P〈0.01,P〈0.05）; two groups of patients with X-ray after treatment of grade m knee synovial fluid IL-1β content also had significant differences （P〈0.05）, while IL-6, INF-et, MMP-3, HA eontent had no significant difference （P〉 0.05）. Conclusion OA-kneeguard can be effectively regulate cytokines, MMP-3, HA biologieal effects, thereby delaying the degeneration of articular cartilage.