液相色谱-串联质谱法测定人血浆中左旋多巴的浓度

Determination of levodopa concentration in human plasma by LC-MS/MS

目的建立测定人血浆中左旋多巴浓度的液相色谱-串联质谱方法。方法采用Agilent公司Eclipse XDB-C18(4.6 mm×150 mm,5μm)色谱柱,流动相为水-甲醇(92∶8,V/V),流速为0.8 mL.min-1;电喷雾(ESI)离子源,正离子电离模式,多反应离子监测的MS扫描方式,左旋多巴和内标的离子通道分别选择为m/z 198.1→m/z 152.0和m/z 154.1→m/z 137.0。每个样品分析时间为3 min。结果左旋多巴在0.01～5 mg.L-1浓度范围内有良好的线性关系,最低定量限为0.01 mg.L-1,日内、日间RSD均小于15%,准确度均在±15%范围内。结论本方法简单、快速、灵敏、准确,可用于人血浆中左旋多巴浓度的检测。

AIM To develop a sensitive and fast liquid chromatography-tandem mass spectrometry method for the determination of levodopa concentration in human plasma. METHODS The column was Eclipse XDB-C18 （4.6 mm×150 mm,5μm）, the mobile phase consisted of water ： methanol （92 ： 8, F/V） and the flow rate was 0.8 mL.min-1. ESI and MRM were used for MS with positive ionization model. The mass transition pairs of m/z 198.1→m/z 152.0 and m/z 154.1→m/z 137.0 were used to detect levodopa and internal standard, respectively. The running time was 3 rain. RESULTS The standard curve of levodopa was linear over the concentration range of 0.01 - 5 mg.L-1. The limit of quantitation of levodopa was 0.01 mg.L-1. Within-day RSD and between-day RSD of levodopa were all less than 15% and the accuracy were all within ± 15%. CONCLUSION The method is simple, fast, sensitive and accurate. It can be used for the determination of levodopa in human plasma.