RANTES及趋化因子受体CCR5在特应性皮炎皮损中的表达及其意义

Expression of RANTES and Chemokine Receptors CCR5 in Patients with Atopic Dermatitis and Its Significance

目的探讨调节正常T细胞表达和分泌活性因子(regulated on activation normal Tcell expressed and secreted,RANTES)及趋化因子受体CCR5在特应性皮炎皮损的表达及其意义。方法采用荧光定量聚合酶链反应法测定20例特应性皮炎患者皮损内及16例正常对照者皮肤内RANTESmRNA及CCR5mRNA的定量表达,采用SCORAD评分评估患者病情,分析特应性皮炎患者皮损内RANTESmRNA及CCR5mRNA的定量表达与SCORAD评分相关性。结果特应性皮炎患者皮损内RANTESmRNA及CCR5mRNA的表达明显高于正常对照组皮肤内的表达(t=10.2,P<0.001;t=2.32,P=0.0318);特应性皮炎患者皮损内RANTESmRNA及CCR5mRNA的定量表达与SCORAD评分均呈显著的正相关(r=0.78465,P<0.0001;r=0.72465,P=0.0003),RANTESmRNA与CCR5mRNA的定量表达呈显著的正相关(r=0.64838,P=0.0020)。结论皮损内RANTES及CCR5的过度表达可能在特应性皮炎的发病中起一定作用。

Objective To investigate the significance and expression of regulated on activation normal T cell expressed and secreted（RANTES） and chemokine receptors CCR5 in patients with atopic dermatitis（AD）. Methods 20 adult patients with AD and 16 health controls were sequentially enrolled in this study. The production of RANTES mRNA and CCR5 mRNA was measured by fluorescent quantitative polymerase chain reaction. The patients＇s condition was measured by SCORAD. The correlation between expression of RANTES mRNA and CCR5 mRNA and SCORAD was analyzed. Results Expression of RANTES mRNA and CCR5 mRNA in AD was significantly higher than that in health controls （t=10.2, P〈0.001; t=2.32, P= 0.0318）. Significant posivtive correlation in AD was found between expression of RANTES mRNA and CCR5 mRNA and SCORAD scores （r=0.78465, P〈0.0001; r=0.72465, P=0.0003）. Significant positive correlation in AD was found between expression of RANTES mRNA and CCR5 mRNA （r=0.64838, P=0.0020）.Conclusion Our results suggest possible role of RANTES and CCR5 in the development of AD.